2020 Modified Biochemistry Lab-- use notes/text to assist :) cloned 9/27/2020 cloned 10/15/2020

Modified Macromolecule Lab... ** NOTE THAT THERE WILL BE NO FEEDBACK ON THIS GOFORMATIVE AND IT IS DUE ON MONDAY, 11/2 BY 10 A.M.

Each of the procedures below can be used to test for organic compounds in a sample:  

Polysaccharides (Iodine Test)

1. Place your sample into a petri dish or test tube.
2. Put 3 to 5 drops of iodine onto your sample.
3. Note the results- black indicates a positive result.

Monosaccharides & Reducing Disaccharides (Benedicts Test)

1. Place your sample in a test tube.  
2. Put 3 to 5 drops of Benedict’s solution onto your sample   
3. Place the test tube into a hot water bath.  
4. Allow the sample to sit in the hot water bath for 2 to 3 minutes.  
5. Carefully remove your test tube with a test tube clamp and note the results- green, yellow and orange indicate a positive result.

Proteins (Biuret Test)
1. Place a your sample in a petri dish or test tube.  
2. Put 3 to 5 drops of Biuret solution onto your sample.  
3. Note the results- violet indicates a positive result.

Lipids (Litmus or Sudan IV Test)
1. Dip your litmus test strip into your sample or add 3-5 drops of Sudan IV reagant.
2. Note the results (light pink= no lipids, red= lipids present)    

Again, how to read positive results:
+ polysaccharides= black
+ protein= dark violet/purple
+ lipids= oil ring (for paper blotter), red color (for Sudan IV litmus test strip)
+ monosaccharides/disaccharides= yellow/orange/green  

1

Let's practice proper hypothesis writing.... choose the BEST answer for this lab. Gelatin, amino acids, glucose, starch and vegetable oil will be tested.

Gelatin:

1

Let's practice proper hypothesis writing.... choose the BEST answer for this lab. Gelatin, amino acids, glucose, starch and vegetable oil will be tested.

Amino Acids:

1

Let's practice proper hypothesis writing.... choose the BEST answer for this lab. Gelatin, amino acids, glucose, starch and vegetable oil will be tested.

Glucose:

1

Let's practice proper hypothesis writing.... choose the BEST answer for this lab. Gelatin, amino acids, glucose, starch and vegetable oil will be tested.

Starch:

1

Let's practice proper hypothesis writing.... choose the BEST answer for this lab. Gelatin, amino acids, glucose, starch and vegetable oil will be tested.

Vegetable oil:

Now, let's review the results...

5

What were the color change results???

Draggable item		Corresponding Item
negative result: no color change		starch solution
positive result: black		vegetable oil solution
positive result: yellow		gelatin solution
positive result: red		glucose solution
positive result: purple		amino acid solution

12

What were the test results??? since vegetable oil was not included with the other biomolecules, it will be excluded in this section of results. *Be sure to indicate all positive and negative results below.

Positive for Biuret (+)
Negative for Iodine (-)
Positive for Benedicts (+)
Positive for Iodine (+)
Negative for Biuret (-)
Negative for Benedicts (-)

gelatin solution
glucose solution
starch solution
amino acid solution

Now, some more foundational practice 🤓 

2

Which test(s) would indicate for the presence of (C6 H12 O6) ?

2

Which test(s) would indicate for the presence of (C12 H22 O11)?

2

Which test(s) would indicate for the presence of (C24 H42 O21)?

1

Which test(s) would indicate for the presence of waxes?
Which test(s) would indicate for the presence of cellulose?
Which test(s) would indicate for the presence of actin/myosin?

Indicate in proper order.

3

Of the organic compounds (starch, glucose, amino acids, vegetable oil, and gelatin) tested for in the video experiment, which were monomers?

2

Of the organic compounds (starch, glucose, amino acids, vegetable oil, and gelatin) tested for in the video experiment, which were polymers?

1

Explain what denaturation is. **Be sure to use your own words- this program flags copied/pasted answers.

1

At what levels can a protein denature?

1

What factors that can affect protein function?

1

What are the FUNCTIONS of fats? Hint... you may want to consult your notes/text.

1

What are the main FUNCTIONS of proteins. Hint... you may want to consult your text/notes.

1

What are the FUNCTIONS of nucleic acids? Hint... you may want to consult your notes/ text.

1

What protein molecule aids in the folding process of proteins? Hint... you may want to consult your notes/textbook.

1

How can misfolding can affect cells... what 3 diseases that may occur as a consequence). Hint.... consult your text!

1

Describe the difference between a glycosidic link and a peptide bond.

1

What is the name of the bond that holds two nucleotides together at their nitrogenous bases?

1

What are the differences between saturated and unsaturated fats in terms of structure and function.

If you need a refresher, here is the Bozeman video on biomolecules :)  Ready to practice specifically with proteins?

Let's practice proper hypothesis writing.... choose the BEST answer for this lab. Gelatin, amino acids, glucose, starch and vegetable oil will be tested.Gelatin:

Let's practice proper hypothesis writing.... choose the BEST answer for this lab. Gelatin, amino acids, glucose, starch and vegetable oil will be tested.Amino Acids:

Let's practice proper hypothesis writing.... choose the BEST answer for this lab. Gelatin, amino acids, glucose, starch and vegetable oil will be tested.Glucose:

Let's practice proper hypothesis writing.... choose the BEST answer for this lab. Gelatin, amino acids, glucose, starch and vegetable oil will be tested.Starch:

Let's practice proper hypothesis writing.... choose the BEST answer for this lab. Gelatin, amino acids, glucose, starch and vegetable oil will be tested.Vegetable oil:

What were the color change results???

What were the test results??? ** since vegetable oil was not included with the other biomolecules, it will be excluded in this section of results. ***Be sure to indicate all positive and negative results below.

Which test(s) would indicate for the presence of (C6 H12 O6) ?

Which test(s) would indicate for the presence of (C12 H22 O11)?

Which test(s) would indicate for the presence of (C24 H42 O21)?

Which test(s) would indicate for the presence of waxes? Which test(s) would indicate for the presence of cellulose? Which test(s) would indicate for the presence of actin/myosin?Indicate in proper order.

Of the organic compounds (starch, glucose, amino acids, vegetable oil, and gelatin) tested for in the video experiment, which were monomers?

Of the organic compounds (starch, glucose, amino acids, vegetable oil, and gelatin) tested for in the video experiment, which were polymers?

Explain what denaturation is. **Be sure to use your own words- this program flags copied/pasted answers.

At what levels can a protein denature?

What factors that can affect protein function?

What are the FUNCTIONS of fats? Hint... you may want to consult your notes/text.

What are the main FUNCTIONS of proteins. Hint... you may want to consult your text/notes.

What are the FUNCTIONS of nucleic acids? Hint... you may want to consult your notes/ text.

What protein molecule aids in the folding process of proteins? Hint... you may want to consult your notes/textbook.

How can misfolding can affect cells... what 3 diseases that may occur as a consequence). Hint.... consult your text!

Describe the difference between a glycosidic link and a peptide bond.

What is the name of the bond that holds two nucleotides together at their nitrogenous bases?

What are the differences between saturated and unsaturated fats in terms of structure and function.

Let's practice proper hypothesis writing.... choose the BEST answer for this lab. Gelatin, amino acids, glucose, starch and vegetable oil will be tested.

Gelatin:

Let's practice proper hypothesis writing.... choose the BEST answer for this lab. Gelatin, amino acids, glucose, starch and vegetable oil will be tested.

Amino Acids:

Let's practice proper hypothesis writing.... choose the BEST answer for this lab. Gelatin, amino acids, glucose, starch and vegetable oil will be tested.

Glucose:

Let's practice proper hypothesis writing.... choose the BEST answer for this lab. Gelatin, amino acids, glucose, starch and vegetable oil will be tested.

Starch:

Let's practice proper hypothesis writing.... choose the BEST answer for this lab. Gelatin, amino acids, glucose, starch and vegetable oil will be tested.

Vegetable oil:

What were the test results??? since vegetable oil was not included with the other biomolecules, it will be excluded in this section of results. *Be sure to indicate all positive and negative results below.

Which test(s) would indicate for the presence of waxes?
Which test(s) would indicate for the presence of cellulose?
Which test(s) would indicate for the presence of actin/myosin?

Indicate in proper order.