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Laabri

Cytochrome C Comparison Lab

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Last updated about 7 years ago
29 Nsɛmmisa
Hyɛ no nsow a efi ɔkyerɛwfo no hɔ:

Ottawa High School Curriculum 2019 Biology textbook McDougal Littell ed 2008

Instructions

Student Worksheet - pg 1 of 2 (open Box #2 to enter numbers into Table 1) We will share data collection. Each student will be given one of the pairs of organisms to count. Data will then be shared.

Student Worksheet - pg 2 of 2

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Asemmisa {{asɛmmisaAhyɛnsode}}
1.

Last Name First Name

enter your name
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2.

Count the number of differences between each of the organisms cytochrome C amino acid sequence and place the number in the appropriate box in the table. See Instructions--> Method--> A--> numbers 1-4 for an example. We will count these as a class and put them on a shared google document. It will be easier for you to enter the data at one time after you have it on the class shared document.

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3.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

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4.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

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5.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

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6.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

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7.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

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8.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

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9.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

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10.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

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11.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

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12.

this is a node, enter the number of differences from your Table 1 between these organisms (#10 and #11)

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13.

this is a node, enter the number of differences from your Table 1 between these organisms

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14.

this is a node, enter the number of differences from your Table 1 between these organisms (#7 &#8)

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15.

this is a node, enter the number of differences from your Table 1 between these organisms (#8 & #9)

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16.

this is a node, enter the number of differences from your Table 1 between these organisms (#6 & #7)

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17.

this is a node, enter the number of differences from your Table 1 between these organisms (#5 & #6)

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18.

this is a node, enter the number of differences from your Table 1 between these organisms (#3 & #5 or #4 & #5); there is more than one correct answer, however, enter only one of the numbers

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19.
Look at the set of amino acid sequences you used to collect data
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20.

enter the animal's name that goes on this branch; use lower case letters

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21.

enter the name of the animal that goes on this branch; use only lower case letters

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22.

enter the name of the organism that belongs on this branch; use only lower case letters

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23.

enter a number

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24.

enter a number

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25.

I used personal knowledge. I have seen a picture of a live horse, donkey and zebra. All of them have similar body structures and look and act "horsey."

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26.

The branch should be added

The fewer the differences in cytochrome C amino acids indicates the organisms are more closely related.
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27.

List 3 things you used to determine how organisms are related to each other.

This lab is an indirect DNA analysis.
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28.

Select whether this a true or false statement to answer Question #6:

More closely related species have more recent common ancestors, therefore there has been less time for DNA mutations to occur and so their DNA is very similar and has less differences.

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29.

Select whether this is a true or false answer to Question #7:

This cytochrome C data indicates that plants and fungi originated off of the same node because they both have similar differences in sequences compared to all of the other animals. Animal life begins off of the next node (more recent) on the cladogram, so both plants and fungi are equal distance from the first node/branch of animals.

Wheat is the plant and yeast is the fungi in this study. All of the other organisms in this study are animals (including the moth). The differences of sequences is in the 40's for both yeast and wheat compared to all of the other organisms.