When a sample gets to the lab for the RT-PCR test, first the genetic material is extracted from the collected cells. Then this mixture consists only of a person's DNA and the Virus RNA (if present). Reverse transcription is completed to "convert" any viral RNA (if present) into DNA. Once this step is complete PCR is used to make thousands of copies of the DNA so that it can be searched more easily for the viral presence. (answer the following and please number your answers accordingly)
1. How is the sample for the RT-PCR test acquired? In other words, how is this test given?
2. What part of Sars CoV-2 does RT-PCR identify the presence of?
3. In our cells, weak hydrogen bonds between base pairs are broken by helicase during DNA replication, in the PCR machine, what is used to break these bonds and separate the strands?
4. What is a con/drawback of the RT-PCR test?