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AGHS - CP Bio - Osmosis Lab

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Osmosis Lab

The goal of this assignment is to help you feel more comfortable with the ideas and vocabulary related to osmosis.

Part 1- Osmosis Simulation

This simulation models osmosis through the cell membrane of ablood cell, an elodea/aquatic plant cell, and a paramecium.

Open this link in a new tab:
http://www.glencoe.com/sites/common_assets/science/virtual_labs/LS03/LS03.html

IF YOU NEED TO ENABLE FLASH, WATCH THIS SCREENCAST SHOWING YOU HOW TO ENABLE FLASH!

Once on this website you should read the explanation on the left side of your screen. Then follow this procedure to complete the data table below:

1. Select one of the 3 cells pictured and drag it into one of the beakers.
2. Observe the process of osmosis. Determine whether water, represented with animated blue arrows, moves into, stays in equilibirum, or moves out of the cell.
3. Record your observations in the table.
4. Move the cell to a different beaker until all the cell has been observed in all solutions types (hypotonic, isotonic, and hypertonic).
5. Repeat the activity until all 3 cells have been observed in all 3 solution types.
6. Answer the obserration and analysis questions below.
Question 1
1.

Question 2
2.

Question 3
3.

Question 4
4.

Question 5
5.

Question 6
6.

Part 2- Red Onion Osmosis Lab


To see how this lab is performed, watch the embedded video. Answer the analysis questions as or after you watch!
Question 7
7.

Question 8
8.

Here is what the red onion cells looked like after the salt solution was drawn across them.
Question 9
9.

Question 10
10.

Question 11
11.

Question 12
12.

The second part of this experiment (starts at 2:16) was to submerge the red onion cells in fresh water using the same technique. This time the cells "swelled back up to their original size" and look like this:
Question 13
13.

Question 14
14.

Here is the second part of the onion lab being run on human red blood cells. Watch all 22 seconds of this video to answer the next two questions!
Question 15
15.

Question 16
16.

Part 3- Applying Osmosis to Real Life Examples

Make sure you participate in the discussion on Schoology to apply your understanding of osmosis, and the accompanying vocabulary, correctly! All instructions are in the "Osmosis Applications" Discussion in todays "Lesson 6- Osmosis Lab" on Schoology.
Based on what you see in the Glencoe animation, sort the cell type in each solution to the situation you observed water moving into the cell, out of it, or showing now net change
paramecium in the hypertonic solution
elodea in the hypertonic solution
paramecium in the isotonic solution
blood cell in the isotonic solution
blood cell in the hypotonic solution
paramecium in the hypotonic solution
elodea in the hypotonic solution
elodea in the isotonic solution
blood cell in the hypertonic solution
Net movement of water into the cell
Net movement of water out of the cell
No net movement of water into or out of the cell
What ALWAYS happens when a cell is placed in a solution that is hypotonic to the cell?
water moves into the cell until an equilibrium is reached
water moves out of the cell until an equilibrium is reached
there is no net movement of water, it enters and exits at the same rate
What ALWAYS happens when a cell is placed in a solution that is hypertonic to the cell?
water moves into the cell until an equilibrium is reached
water moves out of the cell until an equilibrium is reached
there is no net movement of water, it enters and exits at the same rate
What ALWAYS happens when a cell is placed in a solution to which is it isotonic?
water moves into the cell until an equilibrium is reached
water moves out of the cell until an equilibrium is reached
there is no net movement of water, it enters and exits at the same rate
What must be true about the cell membranes of human blood cells, elodea cells, and paramecium is water can easily move across all three cell membranes?
Water can diffuse directly through the phospholipid bilayers of all 3 cell types via simple diffusion.
A pump is present that uses energy to move water into and out of cells
All 3 cell membranes must contain proteins, aquaporins, that specifically allow water to move through the cell membrane
Since water transport occurs from high to low, the diffusion of water across a cell membrane is considered...
active transport
passive transport
Why is it important to use the onion tissue with the red/purple pigment when preapring the slide, not the other side that is completly clear?
the pigment makes the cell walls visible
the pigment makes the cytoplasm visible
they contain a nucleus, nuclei would not exist in plant cells that are clear
What water property allows, or which properties allow, the salt water to move continuously across the onion tissue from one side of the slide where the salt water was added, to the other, where the paper towel was held?
adhesion
cohesion
universal solvent
both adhesion and cohesion
both adhesion and universal solvent
After the cells were bathed in salt water there was a visble change to the cells. Which best describes the events that altered the cells' appearance?
the shape of the cell membrane changed as water was pulled out of the cytoplasm and into the salt solution making the color of the pigment look even darker/more concentrated
the shape of the cell wall changed as water was pulled into the cytoplasm from the salt solution making the color of the pigment look even darker/more concentrated
the shape of the cell membrane changed as water was pulled into the cytoplasm from the salt solution making the color of the pigment look even darker/more concentrated
the shape of the cell wall changed as water was pulled out of the cytoplasm and into the salt solution making the color of the pigment look even darker/more concentrated
Explain the science behind your answer to the previous question? Water was pulled out of the cytoplasm and into the salt solution because...
the water was moving from an area of higher water concentration to an area of lower water concentration
the water was moving from an area of lower water concentration to an area of higher water concentration
Knowing what occurred in this lab, which term best describes the salt solution in relationship to the onion cells when the salt solution was first added to the cells' surrounding environment? The salt solution was ______ compared to the onion cells.
isotonic
hypotonic
hypertonic
What term descibes the appearance of the plant cells when water has been removed from the cytoplasm and there is a visible gap between the cell membrane and the cell walls?
cytolysis
plasmolysis
Knowing what occurred in this lab, which term best describes the fresh water in relationship to the onion cells when the fresh water was first added to the cells' surrounding environment? The fresh water was ______ compared to the onion cells.
isotonic
hypertonic
hypotonic
Since osmosis is a form of passive transport, what should eventually happen to the concentration of water inside of the onion cells compared to the concentration of water in the surrounding solution?
The water concentrations should reach an equilibrium and be isotonic.
The water concentration inside of the cell should increase until it becomes more hypotonic than the water.
The water concentration inside of the cell should increase until it becomes more hypertonic than the water.
How are the results of this test, of adding fresh/distilled water to the surrounding environment of human cells, similar to the results using plant cells?
In both, water moved into the cells because water is hypotonic compared to the cells
In both, water moved out of the cells because water is hypotonic compared to the cells
In both, water moved into the cells because water is hypertonic compared to the cells
In both, water moved out of the cells because water is hypertonic co mparedto the cells
In the video of the human red blood cells being bathed in pure water, we observed cytolysis which literally means the blood cell (cyto) was cut or broken (lysis). Why was this NOT observed in the plant cells found in the onion tissue?
only plant cells have a cytoskeleton to maintain the shape of the cell not animal cells
plant cells can't rupture or be broken because of their cell wall
the phospholipid bilayer in a plant cell is stronger than the phospholipid bilayer in animal cell