Suppose the bases in the DNA above (TAC AAA TAT CGG TTT CCG CAA TCG ATA AGG GGG GCA) were not transcribed correctly and the mRNA now read:
AUG UUU AUA GCC AAG GGC GUU AGC UAU UCC CCC CGU
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Suppose the bases in the DNA above (TAC AAA TAT CGG TTT CCG CAA TCG ATA AGG GGG GCA) were not transcribed correctly and the mRNA now read:
AUG UUU AUA GCC AAA GGC GUA GCU AUU CCC CCC GU
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Question 1
1.
Create a complementary strand to the following DNA strand:
Parent strand - TAC AAA TAT CGG TTT CCG CAA TCG ATA AGG GGG GCA
For the gene we are studying here, this original parent strand will be considered the template strand.
Question 2
2.
What type of strand did you just create? (hint - this is a vocab word)
Question 3
3.
Transcribe the template strand from above to create the mRNA.
Question 4
4.
Translate the mRNA in your answer above into a protein sequence. (you may use the abbreviation for each AA )
For Questions #3 - 7
Question 5
5.
Compare this new mRNA to the normal mRNA in question #1.
How many mistakes were made during transcription?
Question 6
6.
Translate the mRNA above into a protein sequence. (spell each amino acid
correctly and put a dash and no spaces between each).
Question 7
7.
Were any amino acids changed due to any mistakes? If so, which ones?
Question 8
8.
What type of mutation is this? Choose the best answer
For Questions #8 - 12
Question 9
9.
Translate the mRNA above into a protein sequence. (spell each amino acid
correctly and put a dash and no spaces between each).
Question 10
10.
How many amino acids changed due to any mistakes?
Question 11
11.
What group of mutation is this?
Question 12
12.
What type of mutation is this?
Question 13
13.
Which of the two types of mutations had the greatest effect on the protein?
Mutations Diagram For Questions #13 - 17
Question 14
14.
What group of mutations do the diagrams above belong to?
