Lac Operon

Required

8

Draggable item		Corresponding Item

Required

3

What is the function of the repressor protein? How does it carry out its function?

Required

3

Inject 5 lactose molecules into the sim... how does the lactose interact with the repressor protein? How does this interaction regulate the expression of the lacZ gene?

Required

3

How does the lactose interact with the lacZ enzyme (there are really 3 enzymes needed)? What does this interaction supply to the bacterium and then how does it affect its own (lacZ enzyme) activity level?

12

Item 1

Required

8

The trp operon is a(n) __________ operon because it's default state is __________.  This means the bacterium will __________ until __________.  The lac operon is __________ because its default state is __________.  This means the bacterium will __________ until __________.

Required

2

Click on the Lactose Transport tab ... what is the role of the lacY gene?

Required

3

What would happen if there was a mutation in the lacY gene that created a non-functional protein?

Required

3

Explain why natural selection would "create" an repressible operon for tryptophan production and an inducible operon for lactose digestion

Required

5

Why are operons (all of them - inducible and repressible) examples of negative feed back loops/regulation

Required

5

In your own words, summarize how prokaryotes regulate gene expression using operons

10

SKIP '25 - Mutation Challenge - modify the simulation to create various mutations listed in this spreadsheet

Make a copy - fill out the chart using the sim - paste the link to your copy of the chart in the answer box

Draggable item		Corresponding Item
		transcription "switch" for repressor protein
		regulatory gene
		lacZ enzyme (Beta-galactosidase) gene
		transcription "switch" for lacZ enzyme
		where repressor protein binds
		repressor protein
		lacZ enzyme
		inducer

Lac Operon

What is the function of the repressor protein? How does it carry out its function?

Inject 5 lactose molecules into the sim... how does the lactose interact with the repressor protein? How does this interaction regulate the expression of the lacZ gene?

How does the lactose interact with the lacZ enzyme (there are really 3 enzymes needed)? What does this interaction supply to the bacterium and then how does it affect its own (lacZ enzyme) activity level?

Click on the Lactose Transport tab ... what is the role of the lacY gene?

What would happen if there was a mutation in the lacY gene that created a non-functional protein?

Explain why natural selection would "create" an repressible operon for tryptophan production and an inducible operon for lactose digestion

Why are operons (all of them - inducible and repressible) examples of negative feed back loops/regulation

In your own words, summarize how prokaryotes regulate gene expression using operons

SKIP '25 - Mutation Challenge - modify the simulation to create various mutations listed in this spreadsheetMake a copy - fill out the chart using the sim - paste the link to your copy of the chart in the answer box

Go to this pHet simulation - Gene Machine lac operon

What is the function of the repressor protein? How does it carry out its function?

Inject 5 lactose molecules into the sim... how does the lactose interact with the repressor protein? How does this interaction regulate the expression of the lacZ gene?

How does the lactose interact with the lacZ enzyme (there are really 3 enzymes needed)? What does this interaction supply to the bacterium and then how does it affect its own (lacZ enzyme) activity level?

Click on the Lactose Transport tab ... what is the role of the lacY gene?

What would happen if there was a mutation in the lacY gene that created a non-functional protein?

Explain why natural selection would "create" an repressible operon for tryptophan production and an inducible operon for lactose digestion

Why are operons (all of them - inducible and repressible) examples of negative feed back loops/regulation

In your own words, summarize how prokaryotes regulate gene expression using operons

SKIP '25 - Mutation Challenge - modify the simulation to create various mutations listed in this spreadsheetMake a copy - fill out the chart using the sim - paste the link to your copy of the chart in the answer box

SKIP '25 - Mutation Challenge - modify the simulation to create various mutations listed in this spreadsheet

Make a copy - fill out the chart using the sim - paste the link to your copy of the chart in the answer box

SKIP '25 - Mutation Challenge - modify the simulation to create various mutations listed in this spreadsheet

Make a copy - fill out the chart using the sim - paste the link to your copy of the chart in the answer box