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Biblioteka

DNA Analysis Practice

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Posljednje ažuriranje almost 4 years ago
4 questions

Restriction enzyme HpyCH4 V cuts DNA fragments at the sequence TGCA between the G and C, as shown in the image.

1
1
1

The steps of gel electrophoresis are below. They are not in the correct order.

  1. Load the DNA into the wells of the gel. Include a DNA control for comparison.

  2. Watch as the smaller DNA fragments move toward the positive end of the gel.

  3. Cut DNA into fragments with restriction enzymes

  4. Connect the gel chamber to an electrical current.

  5. Stain the DNA bands to make them easily visible.

1
Pitanje 1
1.

How many "cuts" will be made using this enzyme on the following DNA sequence: TGCAAAAGGTGCATTCGTGCA

Pitanje 2
2.

What is used to cut long strands of DNA into smaller fragments?

Pitanje 3
3.

Which of the following is true about gel electrophoresis?

Pitanje 4
4.

Using the steps above, what is the correct order of the steps for creating a DNA fingerprint?