Microscope Pre-Lab A&P (shared)

Take notes over the video and prepare to answer some questions.

Required

20

Upload a photo of your notes.

REMINDER: Copy and pasted answers will not be accepted.

Purpose:
In this pre-lab you will:
Identify the parts of the microscope and their uses
Learn safe microscope operations
Prepare to use the microscope in lab next class period
Click the following link to go to the Virtual Microscope Lab.  When you arrive, click “Launch Activity” to enter the laboratory.  This virtual microscope is VERY similar to the actual microscope that you will be using in class next class period.

Required

10

Match the part of the microscope with its location on the diagram

Draggable item		Corresponding Item
objective lens		C24
mechanical stage		C12
base		C8
coarse adjustment knob		C10
ocular (eyepiece)		C4
Abbe condenser		C2
diaphragm		C14
head		C13
rheostat (dimmer)		C23
light source		C18b
fine adjustment knob		C22

Required

10

Match the microscope structure with its function

Draggable item		Corresponding Item
ocular (eyepiece)		Moves stage up and down in large increments (DO NOT use it on high power above 10x)
Abbe condenser		Moves stage up and down in small increments. Use after coarse adjustment. (If using the 40x or 100x objectives it is the ONLY one you use)
base		Holds and positions the slide
diaphragm		Adjusts intensity of light
objective lenses		Controls amount of light that can reach specimen by changing diameter of opening
coarse adjustment knob		Raises or lowers the diaphragm and thereby changes angle of light and intensity
rheostat (dimmer)		The part you look through! Usually magnify 10x or 25x
mechanical stage		Magnifying lenses (4x, 10x, 40x, 100x (oil immersion lens)
fine adjustment knob		Lowest part of the microscope; supports the rest

Part 2: Questions
Before you can begin observing specimens, you should familiarize yourself with the parts of the microscope and their functions.  Click “Guide” at the bottom of the screen, then select “Overview”.

Link to Virtual Microscope 

Required

4

What is the proper way to carry a microscope?

Required

4

What happens when the revolving nose-piece is rotated?

Now click “Objective Lenses”.

Required

4

How can you determine the magnification of the ocular lens on your microscope?

Required

10

Match the color of the objective lens with its magnification.

Draggable item		Corresponding Item
red		40x (high power)
yellow		10x (low power)
white		4x (scanning)
blue		100x (oil immersion)

Required

4

If the ocular lens has a magnifying power of 25X, and the low power objective lens is being used, what is the total magnification of the microscope? Show your work!

Required

4

Now click “Microscope Care”.
Why must you never touch the lens of a microscope with your fingers?

Now close the reference guide and click “Learn” on the main page.  Explore the parts of the microscope by clicking on each question mark, and answer the following questions.

Required

4

Under what circumstances would immersion oil be used?

Required

4

If you need to move the stage to the left, which part would be used?

Required

4

What is the difference between the coarse focus (adjustment knob) and the fine focus?

Required

4

The ocular lens of your microscope has several smudges. What would you use to clean the lens: paper towels or lens paper? Why?

Required

4

Now click “Next”.

If you were trying to simply locate a grouping of cells under the microscope, which lens would be used? Explain using the term field of view.

Required

10

Use Chapter 3 in the Online Textbook to help you define the following terms:

Draggable item		Corresponding Item
specimen		The thing on the slide that you are observing.
resolution		Ability of a lens to resolve fine details of an observed object.
micrometer (µm)		Space between the objective lens and the specimen
parfocal		Metric linear measurement used in microscopy.
working distance		A microscope that is calibrated this way will remain mostly in focus when the nosepiece is rotated and the next highest objective is moved into position.

Microscope Pre-Lab A&P (shared)

Upload a photo of your notes.

Match the part of the microscope with its location on the diagram

Match the microscope structure with its function

What is the proper way to carry a microscope?

What happens when the revolving nose-piece is rotated?

How can you determine the magnification of the ocular lens on your microscope?

Match the color of the objective lens with its magnification.

If the ocular lens has a magnifying power of 25X, and the low power objective lens is being used, what is the total magnification of the microscope? Show your work!

Now click “Microscope Care”.Why must you never touch the lens of a microscope with your fingers?

Under what circumstances would immersion oil be used?

If you need to move the stage to the left, which part would be used?

What is the difference between the coarse focus (adjustment knob) and the fine focus?

The ocular lens of your microscope has several smudges. What would you use to clean the lens: paper towels or lens paper? Why?

Now click “Next”.If you were trying to simply locate a grouping of cells under the microscope, which lens would be used? Explain using the term field of view.

Use Chapter 3 in the Online Textbook to help you define the following terms:

Upload a photo of your notes.

Match the part of the microscope with its location on the diagram

Match the microscope structure with its function

What is the proper way to carry a microscope?

What happens when the revolving nose-piece is rotated?

How can you determine the magnification of the ocular lens on your microscope?

Match the color of the objective lens with its magnification.

If the ocular lens has a magnifying power of 25X, and the low power objective lens is being used, what is the total magnification of the microscope? Show your work!

Now click “Microscope Care”.Why must you never touch the lens of a microscope with your fingers?

Under what circumstances would immersion oil be used?

If you need to move the stage to the left, which part would be used?

What is the difference between the coarse focus (adjustment knob) and the fine focus?

The ocular lens of your microscope has several smudges. What would you use to clean the lens: paper towels or lens paper? Why?

Now click “Next”.If you were trying to simply locate a grouping of cells under the microscope, which lens would be used? Explain using the term field of view.

Use Chapter 3 in the Online Textbook to help you define the following terms:

Now click “Microscope Care”.
Why must you never touch the lens of a microscope with your fingers?

Now click “Next”.

If you were trying to simply locate a grouping of cells under the microscope, which lens would be used? Explain using the term field of view.

Now click “Microscope Care”.
Why must you never touch the lens of a microscope with your fingers?

Now click “Next”.

If you were trying to simply locate a grouping of cells under the microscope, which lens would be used? Explain using the term field of view.