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Microscope Pre-Lab A&P (shared)

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Last updated over 3 years ago
15 questions
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Take notes over the video and prepare to answer some questions.
Question 1
1.

Upload a photo of your notes.

REMINDER: Copy and pasted answers will not be accepted.
Purpose:
In this pre-lab you will:
  • Identify the parts of the microscope and their uses
  • Learn safe microscope operations
  • Prepare to use the microscope in lab next class period
Click the following link to go to the Virtual Microscope Lab. When you arrive, click “Launch Activity” to enter the laboratory. This virtual microscope is VERY similar to the actual microscope that you will be using in class next class period.
Question 2
2.

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Question 3
3.

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Part 2: Questions
Before you can begin observing specimens, you should familiarize yourself with the parts of the microscope and their functions. Click “Guide” at the bottom of the screen, then select “Overview”.

Link to Virtual Microscope
Question 4
4.

What is the proper way to carry a microscope?

Question 5
5.

What happens when the revolving nose-piece is rotated?

Now click “Objective Lenses”.
Question 6
6.

How can you determine the magnification of the ocular lens on your microscope?

Question 7
7.

Match the color of the objective lens with its magnification.

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white
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40x (high power)
red
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10x (low power)
yellow
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Question 8
8.

If the ocular lens has a magnifying power of 25X, and the low power objective lens is being used, what is the total magnification of the microscope? Show your work!

Question 9
9.

Now click “Microscope Care”.
Why must you never touch the lens of a microscope with your fingers?

Now close the reference guide and click “Learn” on the main page. Explore the parts of the microscope by clicking on each question mark, and answer the following questions.
Question 10
10.

Under what circumstances would immersion oil be used?

Question 11
11.

If you need to move the stage to the left, which part would be used?

Question 12
12.

What is the difference between the coarse focus (adjustment knob) and the fine focus?

Question 13
13.

The ocular lens of your microscope has several smudges. What would you use to clean the lens: paper towels or lens paper? Why?

Question 14
14.

Now click “Next”.

If you were trying to simply locate a grouping of cells under the microscope, which lens would be used? Explain using the term field of view.

Question 15
15.

Use Chapter 3 in the Online Textbook to help you define the following terms:

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specimen
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The thing on the slide that you are observing.
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Match the part of the microscope with its location on the diagram
coarse adjustment knob
C24
fine adjustment knob
C12
diaphragm
C8
ocular (eyepiece)
C10
rheostat (dimmer)
C4
head
C2
objective lens
C14
base
C13
mechanical stage
C23
Abbe condenser
C18b
light source
C22
Match the microscope structure with its function
mechanical stage
Moves stage up and down in large increments (DO NOT use it on high power above 10x)
diaphragm
Moves stage up and down in small increments. Use after coarse adjustment. (If using the 40x or 100x objectives it is the ONLY one you use)
rheostat (dimmer)
Holds and positions the slide
coarse adjustment knob
Adjusts intensity of light
ocular (eyepiece)
Controls amount of light that can reach specimen by changing diameter of opening
base
Raises or lowers the diaphragm and thereby changes angle of light and intensity
Abbe condenser
The part you look through! Usually magnify 10x or 25x
objective lenses
Magnifying lenses (4x, 10x, 40x, 100x (oil immersion lens)
fine adjustment knob
Lowest part of the microscope; supports the rest
4x (scanning)
blue
100x (oil immersion)
working distance
Ability of a lens to resolve fine details of an observed object.
parfocal
Space between the objective lens and the specimen
micrometer (µm)
Metric linear measurement used in microscopy.
resolution
A microscope that is calibrated this way will remain mostly in focus when the nosepiece is rotated and the next highest objective is moved into position.