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Microscope Pre-Lab A&P (shared)

Last updated about 3 years ago
15 questions
Take notes over the video and prepare to answer some questions.
Required
20

Upload a photo of your notes.

REMINDER: Copy and pasted answers will not be accepted.
Purpose:
In this pre-lab you will:
  • Identify the parts of the microscope and their uses
  • Learn safe microscope operations
  • Prepare to use the microscope in lab next class period
Click the following link to go to the Virtual Microscope Lab. When you arrive, click “Launch Activity” to enter the laboratory. This virtual microscope is VERY similar to the actual microscope that you will be using in class next class period.
Required
10

Match the part of the microscope with its location on the diagram

Draggable itemCorresponding Item
light source
C24
coarse adjustment knob
C12
base
C8
fine adjustment knob
C10
ocular (eyepiece)
C4
head
C2
diaphragm
C14
objective lens
C13
Abbe condenser
C23
rheostat (dimmer)
C18b
mechanical stage
C22
Required
10

Match the microscope structure with its function

Draggable itemCorresponding Item
coarse adjustment knob
Moves stage up and down in large increments (DO NOT use it on high power above 10x)
objective lenses
Moves stage up and down in small increments. Use after coarse adjustment. (If using the 40x or 100x objectives it is the ONLY one you use)
base
Holds and positions the slide
ocular (eyepiece)
Adjusts intensity of light
fine adjustment knob
Controls amount of light that can reach specimen by changing diameter of opening
rheostat (dimmer)
Raises or lowers the diaphragm and thereby changes angle of light and intensity
mechanical stage
The part you look through! Usually magnify 10x or 25x
diaphragm
Magnifying lenses (4x, 10x, 40x, 100x (oil immersion lens)
Abbe condenser
Lowest part of the microscope; supports the rest
Part 2: Questions
Before you can begin observing specimens, you should familiarize yourself with the parts of the microscope and their functions. Click “Guide” at the bottom of the screen, then select “Overview”.

Link to Virtual Microscope
Required
4

What is the proper way to carry a microscope?

Required
4

What happens when the revolving nose-piece is rotated?

Now click “Objective Lenses”.
Required
4

How can you determine the magnification of the ocular lens on your microscope?

Required
10

Match the color of the objective lens with its magnification.

Draggable itemCorresponding Item
red
40x (high power)
blue
10x (low power)
yellow
4x (scanning)
white
100x (oil immersion)
Required
4

If the ocular lens has a magnifying power of 25X, and the low power objective lens is being used, what is the total magnification of the microscope? Show your work!

Required
4

Now click “Microscope Care”.
Why must you never touch the lens of a microscope with your fingers?

Now close the reference guide and click “Learn” on the main page. Explore the parts of the microscope by clicking on each question mark, and answer the following questions.
Required
4

Under what circumstances would immersion oil be used?

Required
4

If you need to move the stage to the left, which part would be used?

Required
4

What is the difference between the coarse focus (adjustment knob) and the fine focus?

Required
4

The ocular lens of your microscope has several smudges. What would you use to clean the lens: paper towels or lens paper? Why?

Required
4

Now click “Next”.

If you were trying to simply locate a grouping of cells under the microscope, which lens would be used? Explain using the term field of view.

Required
10

Use Chapter 3 in the Online Textbook to help you define the following terms:

Draggable itemCorresponding Item
specimen
The thing on the slide that you are observing.
working distance
Ability of a lens to resolve fine details of an observed object.
micrometer (µm)
Space between the objective lens and the specimen
resolution
Metric linear measurement used in microscopy.
parfocal
A microscope that is calibrated this way will remain mostly in focus when the nosepiece is rotated and the next highest objective is moved into position.