use with flow of genetic information kit
Use with the 3-D Molecular Designs 'Flow of Genetic Information Kit'.
Students should be modeling replication with the foam pieces and answering the questions in this formative.
List at least three reasons why a cell must undergo division.
Imagine you are cutting a bagel (one of the most common household injuries) and you get a cut. The cut heals. How do the new cells compare to the original (pre-cut) cells?
Record the template strand bases. Start at the 3' end. Do not leave spaces between the nucleotides.
Examine the strands of DNA. What do you observe about the arrow ends of the model?
What is attached to the 5' Carbon?
The blank on the left is the ___ carbon and the blank on the right is the ___ carbon
Find, circle, and label the 5’ carbon of BOTH nucleotide 1 and nucleotide 4 in the diagram.
Find, circle, and label the 3’ carbon of nucleotide 3 and nucleotide 6 in the diagram.
What functional group is attached to the 3’ carbon? What group is attached to the 5’ carbon?
Circle the replication bubble and draw arrows pointing to the replication forks in the model.
Looking at the mini toober (flexible foam tubes) model, what do you think might be the first step of replication?
Nucleotides are added at an approximate rate of 50 nucleotides per second in eukaryotic cells. The human genome contains 6.4 billion nucleotides (3.2 billion base pairs), which must be copied. Calculate the length of time in days that it would take to copy the human genome. Show all calculations including units. There is a hint available.
As a new nucleotide is added to the growing DNA strand, which part of the new nucleotide forms a bond with the 3’ OH (hydroxyl) group?
Draw an arrow indicating the directionality of the newly replicated leading strand of DNA.
Sketch and indicate the directionality of the fragments composing the lagging strand of DNA.
Why is DNA replication considered to be a semi-discontinuous process?
When you are satisfied that you know how to answer this question ask your teacher for a paper copy of this question.
How does your body ensure that the new cells are the same?
How does DNA get into the new cells?
What does the arrow indicate?
How are the 3’ and 5’ carbons oriented in the strands of the DNA molecule you assembled?
Why do you think multiple replication bubbles form during the process of DNA replication?
What does the helicase appear to be doing?
Identify which type of bond is broken.
What part of the helicase enzyme is represented by the pointed orange wedge?
Why won't you be able to synthesize the other strand of DNA in a continuous manner when using the model?
How do these two new strands compare to the original (parental) strand? (may be more than 1 correct answer)
