Translation Activity (Flow of Genetic Information Kit)

Last updated 4 months ago

28 questions

The expectation is that you and your partners work together to complete this activity.  Read all of the directions.  Discuss all of the questions.  Ask for help if you need it.

Required

At this rate, how many seconds would it take to make a protein such as actin which is 375 amino acids long?

Required

Draggable item		Corresponding Item
UAG		met
AUA		cys
CAU		glu
UGG		Ile
AGA		his
GAG		trp
AUG		pro
CCA		arg
UGU		stop

Draggable item		Corresponding Item
CAU		UAC
CCA		ACA
UGU		CUC
UAG		UAU
AUA		GUA
CAC		ACC
AUG		GGU
GAG		UCU
AGA		GUG
UGG		AUC

Translation Activity (Flow of Genetic Information Kit)

Translation Activity (Flow of Genetic Information Kit)

At this rate, how many seconds would it take to make a protein such as actin which is 375 amino acids long?

Identify the amino acid that is joined to the 3’ end of the tRNA

Identify the anticodon of the tRNA.

At this rate, how many seconds would it take to make a protein such as actin which is 375 amino acids long?

What part of the mRNA nucleotide contains the information to make a protein?

Identify the three-letter abbreviation for each amino acid in the table: (note - I skipped codon 9 in this question)

Identify the amino acid that is joined to the 3’ end of the tRNA

Identify the anticodon of the tRNA.

What amino acid is associated with the tRNA that will bind to the mRNA start codon AUG?

Record the tRNA anticodons that are complementary to each of the mRNA codons

Discuss the answer to this question with your partners. We discussed this question and...

Which end of the mRNA strand attaches to the small ribosomal subunit?

Referring to the previous amino acid codon table you completed (see Q7), record which tRNA anticodon will attach first in this P site.

Which tRNA-amino acid complex will attach into the A site at this time?

Identify the peptide bond in the photo to the right.

The tRNA is easily separated from mRNA because they are held together by

Discuss the benefits of recycling the tRNA with your partners.

Which mRNA codon is now located in the A site?

Which tRNA need to be “recycled”? (you used the same tRNA twice when building your polypeptide)

Why did the tRNA for the second histidine NOT need to be recycled?

What occurs at the mRNA codon UAG?

Using The Genetic Codon Chart© included in the kit or the one at the end of the handout, identify the various stop codons.

What is the order of amino acids in your polypeptide? Start with Met at the top.

Compare the amino acid sequence of the polypeptide you created to the sequence predicted in question 7. How do your sequences compare?

Discuss what happens to the polypeptide as the mRNA strand is being translated with your partners.

What will happen next to the polypeptide? Select all correct answers

As you have followed this process of translation, what steps are now left to be completed? What will happen to the various molecules at the end of this process?

Discuss this question with your partners: How long did this process of translation take for you and your lab group? Do you think the cell could operate at this rate?

Discuss the entire process of transcription and translation with your lab group. Record your answers in your notebook. Which part would your group like to present to the class?

Draggable item		Corresponding Item
CAU		UAC
CCA		ACA
UGU		CUC
UAG		UAU
AUA		GUA
CAC		ACC
AUG		GGU
GAG		UCU
AGA		GUG
UGG		AUC

Draggable item		Corresponding Item
CAU		UAC
CCA		ACA
UGU		CUC
UAG		UAU
AUA		GUA
CAC		ACC
AUG		GGU
GAG		UCU
AGA		GUG
UGG		AUC

Draggable item		Corresponding Item
CAU		UAC
CCA		ACA
UGU		CUC
UAG		UAU
AUA		GUA
CAC		ACC
AUG		GGU
GAG		UCU
AGA		GUG
UGG		AUC