DNA Warm-up

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What do you already know about DNA? Bullet points are fine.

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Type the complementary DNA strand for the segment shown.
_______ 

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Select all of the base pairs in the following image that violate Chargaff's Rules.

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SLOPP-Y is a restriction enzyme that cuts anywhere it encounters a CA sequence in between the C and the A.

Draw a line anywhere the restriction enzyme SLOPP-Y will cut! Remember to read the bottom strand from right to left.

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Record the class data in the data table and then create a bar graph of your data below!

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What is the role of restriction enzymes in DNA profiling?

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Show how the following DNA sequence would be cut by the restriction enzyme Hae III. Count the number of base pairs in each fragment and put a label at the top of each fragment.

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What is polymerase chain reaction (PCR) used for?

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The AMEL marker indicates the _______ of the individual. 

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Construct a Punnett Square for the scenario shown in the figure.

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Examine the STR profiles below. Which suspect's DNA profile is consistent with the crime scene STR profile? __________

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D7S280 is an STR found on human chromosome 7 that repeats the sequence GATA. The DNA sequence of the representative allele of this locus is shown. Find the repeat sequence GATA. How many repeats are shown on the DNA sequence? Different alleles of this locus may have from 6 to 15 tandem repeats of GATA.

_______ repeats

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One of the hotel guests that was not accounted for after the fire was a tourist, John Doe, visiting Reno from New York. Authorities sampled DNA from his toothbrush obtained from his apartment in New York. They used the DNA to generate a DNA profile using the 13 standard STR loci. The data is shown in Figure 2.

The population of the United States in 2006 was about 298.4 million (2.98 × 108). Based on this information, explain why the medical examiner was confident in telling the victim’s family that the recovered remains were those of their son.

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Which of the following options is not a potential source of DNA?

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List 2 precautions the officer is taking to prevent contamination.

Background
Anna Anderson claimed for more than 60 years that she was Princess Anastasia, the Grand Duchess of Russia, daughter of Tsar Nicholas and Tsarina Alexandra. Anna Anderson knew a great deal about life in the Russian palace and she could speak very knowledgably about family members. Anna had many people convinced that she was Anastasia, but does the DNA evidence support her claim?

In this activity, you will examine the STR profiles of Anna Anderson, Tsar Nicholas, and Tsarina Alexandra to determine if the DNA evidence supports the claim that Anna Anderson was the child of the tsar and tsarina. Recall that each parent must contribute one STR allele for each STR marker.

What do you already know about DNA? Bullet points are fine.

Select all of the base pairs in the following image that violate Chargaff's Rules.

SLOPP-Y is a restriction enzyme that cuts anywhere it encounters a CA sequence in between the C and the A.Draw a line anywhere the restriction enzyme SLOPP-Y will cut! Remember to read the bottom strand from right to left.

Record the class data in the data table and then create a bar graph of your data below!

What is the role of restriction enzymes in DNA profiling?

Show how the following DNA sequence would be cut by the restriction enzyme Hae III. Count the number of base pairs in each fragment and put a label at the top of each fragment.

What is polymerase chain reaction (PCR) used for?

Construct a Punnett Square for the scenario shown in the figure.

Which of the following options is not a potential source of DNA?

List 2 precautions the officer is taking to prevent contamination.

Background

SLOPP-Y is a restriction enzyme that cuts anywhere it encounters a CA sequence in between the C and the A.

Draw a line anywhere the restriction enzyme SLOPP-Y will cut! Remember to read the bottom strand from right to left.