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Bacteria Exit Ticket Part 4 - Antibiotic Resistance

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Last updated 2 months ago
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Which antibiotic (A, B, or C) is this bacterium resistant to and explain how the image allows you to figure this out?

What is a biofilm primarily composed of?
Only bacteria
Microorganisms (bacteria) and an extracellular matrix (slime)
Just human cells
Only DNA fragments
What are the parts of a biofilm?
bacteria
"slime" - polysaccharides made by the bacteria
human cells
antibodies
Where are biofilms commonly found?
anywhere that is moist
On clean surfaces
Only in laboratories
Only in dry area
What purpose do biofilms serve for bacteria?
allows bacteria to travel to new environments
Oxygen production
gives ability to reproduce with viruses
Protection from outside and access to nutrients
provides an environment where bacteria can be close to each other and share DNA by conjugation
How do biofilms contribute to antibiotic resistance?
Reduce genetic mutations
Accelerate drug metabolism
Break down antibiotics
Shield bacteria from antibiotics from outside
allow bacteria to easily share/trade plasmids through conjugation that may have genes for antibiotic resistance
Which environment promotes biofilm formation?
Open air
Dry and sterile conditions
Moist and nutrient-rich areas
High UV light exposure
Biofilms are typically easy to remove permamently from surfaces
True
False
Does penicillin kills bacteria by poking holes in their cell walls? Watch the video ...
true
false
Look at the timeline of antibiotic research. Humans just recently invented antibiotics in the last few years.... true or false?
True
False
New antibiotics are still be researched and developed .... true or false?
True
False
According to the diagram, what the mechanisms that do bacteria use to resist (fight off) antibiotics?

inactivate the antibiotic by using their enzymes
stopping the uptake of the antibiotic into their cells
pumping out the antibiotic using an efflux pump
altering the target of the antibiotic by creating a modified drug target that "grabs" onto the antibiotic
How could an antibacterial resistance trait spread through a bacteria population WITHOUT the bacteria needing to reproduce?

good luck
many mutations
bacteria copying and sharing plasmids with each other
resistant bacteria conjugating with other bacteria in their population
Antibiotic Resistance - Put the following steps in order from first event to last event
Resistant bacteria frequently share antibiotic resistance genes by conjugation - this makes antibiotic resistance spread even faster!
An antibiotic enters the environment of a population of bacteria.
The resistance bacteria are “selected for”. They survive to reproduce...
Most of the bacteria are killed - the resistant bacteria are not!
The resistant bacteria reproduce successfully and the amount of resistant bacteria increases in the population
Some mutations (rarely) may allow a bacterium to RESIST (fight off) an antibiotic.
Mutations - random mistakes in copying bacterial DNA as bacterium gets ready for binary fission (to divide)
A population of resistant (or mostly) resistant bacteria has been created.
What is multi-drug resistant bacteria ("superbug")?
a strain of bacterium that is resistant to several or many antibiotics
a bacterium that is able to make its own antibiotics
a bacterium that has the ability to reproduce very quickly

From "Superbugs That Resist Antibiotics Can Evolve in 11 Days | I Contain Multitudes", According to the video, antibiotics are natural substances that have been in use for billions of years. True or False?

a. True
b. False

From "Superbugs That Resist Antibiotics Can Evolve in 11 Days | I Contain Multitudes", What did Alexander Fleming observe in his lab that led to the discovery of penicillin?

a. A mold that had killed bacteria around it
b. A new type of bacteria
c. A chemical reaction between two substances
d. A virus that infected bacteria

From "Superbugs That Resist Antibiotics Can Evolve in 11 Days | I Contain Multitudes", What is the main concern with the overuse of antibiotics?

a. It can cause antibiotic resistance in bacteria
b. It can lead to allergic reactions in patients
c. It can be expensive for patients
d. It can have negative side effects on the immune system

From "Superbugs That Resist Antibiotics Can Evolve in 11 Days | I Contain Multitudes", How long did it take for the population of E. coli on the Mega-Plate to evolve super resistance to the antibiotic?

a. 5 days
b. 7 days
c. 11 days
d. 14 days

From "Superbugs That Resist Antibiotics Can Evolve in 11 Days | I Contain Multitudes", What does the video suggest about the use of antibiotics to treat viral diseases?

a. It is highly effective and recommended
b. It can help relieve symptoms temporarily
c. It is useless and can make matters worse
d. It can prevent bacterial infections from occurring
Antibiotics are not only given to people, but to farm animals as well. Antibiotics are also put into many cleaners and detergents. Our environment is full of antibiotics!



Watch this video from the beginning to 5:40 in. You do not have to watch after 5:40.

Why are farm animals fed antibiotics?
to keep them from getting sick on factory farms where they live VERY close to each other
to help the animals grow more quickly
to stop them from misbehaving
to make sure they cannot get infected by a virus
According to the chart, what is the largest percent usage of antibiotics in the US?
in human medicine - to fight bacterial infections
as preventive medicine in our farm animals and to help farm animals grow more quickly
Watch this video called "Microbiology resources - How to Measure Zones of Inhibition (ZOI)"



Then watch this short video ....




Describe & explain what is different between the bacterial growth on the 2 petri dishes below
The left plate has an antibiotic disc added to the center which has create a "kill zone" around the disc. The right disc shows an undisturbed lawn of bacteria
The right plate has an antibiotic disc added to the center which has create a "kill zone" around the disc. The left disc shows an undisturbed lawn of bacteria
Which antibiotic is most effective at killing this type of bacterium?
A
B
C