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2006/L18- Introduction to cell cycle

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Last updated about 2 months ago
15 questions
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Question 1
1.

Which of the following is the most critical function of the G1 phase in the eukaryotic cell cycle

Question 2
2.

During which checkpoint is the activation of APC/C most critical?

Question 3
3.

What would be the most likely outcome for a cell that cannot repair severe DNA damage and cannot proceed through the G2/M checkpoint?

Question 4
4.

Which of the following events occurs only once per cell cycle and must be strictly regulated?

Question 5
5.

In budding yeast, which of the following experimental tools is primarily used to study essential cell cycle genes?

Question 6
6.

A fission yeast mutant with defective Rad9 protein fails to pause in G2 after DNA damage. What does this indicate about Rad9’s role?

Question 7
7.

What is the primary purpose of the spindle assembly checkpoint (SAC)?

Question 8
8.

Which pair of model organisms has been most influential in identifying Cdk1/Cdc2 activity?

Question 9
9.

If a mutation in a cell prevents degradation of Cyclin B, what phase transition is most likely to be affected?

Question 10
10.

Which best explains why synchronous cells are preferred for biochemical studies of the cell cycle?

Question 11
11.

A researcher adds progesterone to Xenopus oocytes. What immediate response should be expected?

Question 12
12.

Which is the best explanation for why uncontrolled progression through the cell cycle is dangerous?

Question 13
13.

In which cell cycle phase are centrosomes duplicated?

Question 14
14.

What is a defining structural feature of a chromosome that enables attachment of spindle fibers during mitosis?

Question 15
15.

Which checkpoint ensures that environmental signals and DNA integrity are appropriate before a cell commits to a new cycle?

Assessment of cellular conditions before DNA synthesis
Apoptosis
Metaphase-anaphase transition
S-phase checkpoint
Apoptosis or permanent withdrawal from the cell cycle
Immediate differentiation
Formation of tetraploid cells
Cytokinesis
Chromosome segregation
DNA replication
Temperature-sensitive (ts) mutants
Site-directed mutagenesis
Fluorescent tagging of histones
It is essential for spindle attachment
It acts as part of the DNA damage checkpoint
It enhances apoptotic pathways
To ensure chromosomes are properly attached to the mitotic spindle
To detect chromosome condensation errors
To activate cyclin synthesis
Xenopus eggs and Chinese Hamster Ovary cells
Mouse embryonic stem cells and zebrafish
E. coli and yeast
Metaphase to anaphase
Telophase to G1
They provide uniformity in cell cycle phase for clearer analysis
They are easier to genetically manipulate
They produce higher protein yields
Progression through G2/M transition
Induction of apoptosis
It can lead to genomic instability and cancer
It enhances immune cell activation
It increases differentiation rates
G2
M
Kinetochores at the centromere
Histone core
S-phase replication checkpoint
G1/S restriction checkpoint
Anaphase promoting complex checkpoint