A four-function or scientific calculator must be available for you to use while taking this examination. Note that diagrams are not drawn to scale unless otherwise noted.
A four-function or scientific calculator must be available for you to use while taking this examination. Note that diagrams are not drawn to scale unless otherwise noted.
Base your answers to questions 1 through 5 on the information below and on your knowledge of biology.
Two Kinds of Twins
Twins are two offspring produced by the same pregnancy. The most common types of twins are identical twins and fraternal twins. The genes of fraternal twins may vary as much as non-twin siblings, whereas identical twins share the same DNA. However, as identical twins age, their physical characteristics can show differences that distinguish them from each other.
The models below represent some information about twin development.

Use the information in the models to identify which model (A or B) represents fetuses that would continue to develop into identical twins and identify one or more structures in the model that you selected that could be used to support your answer. Include your response for:
Model:
Structure(s):
Use evidence and reasoning to defend the claim that processes that occur during sexual reproduction result in fraternal twins having different traits.
Scientists are investigating how the expression of genes in identical twins may be altered by epigenetic changes. Epigenetic changes occur when certain chemical groups, such as methyl groups, attach to a cell’s DNA and influence the expression of particular genes. Environmental factors and lifestyle choices such as diet have been linked to epigenetic changes. The model below shows how methyl groups can attach to DNA.

Electrophoresis is a process that can be used to compare DNA segment migration through a gel. The image below shows a comparison of DNA taken from body cells of 3-year-old identical twins and 50-year-old identical twins.

A researcher wants to determine the cause of the amount of variation seen between identical twins at 50 years old when compared to twins at 3 years old. Which question should they ask to help them determine the cause?
Base your answers to questions 6 through 9 on the information below and on your knowledge of biology.
Space Invader
Hydrilla is an invasive aquatic plant with an extremely high growth rate. It can grow up to 2.5 cm per day, producing thick mats in waterways. Hydrilla has the same access to resources needed to carry out photosynthesis as native plants such as pondweed, but it invades their space, obtaining available resources faster and more efficiently.
The thick mats that Hydrilla forms are caused by long stems that branch at the surface of the water. The long stems can easily be broken off the parent plant by actions like water movement, animal activity, or human recreation, such as boating. A small piece of Hydrilla can establish a new population.
Hydrilla Mat

Complete the model by identifying the inputs and outputs of the process used by Hydrilla to outcompete native plants.

Inputs
• ______
• ______
Outputs
• _____
• _____
Base your answers to questions 10 through 13 on the information below and on your knowledge of biology.
Keystone Species Rule
Some organisms play a disproportionately large role in an ecosystem. The presence or absence of these species has a very large impact on the rest of the ecosystem and how it functions. These organisms are known as keystone species. The impact of these changes is known as a trophic cascade and can occur from the top-down or the bottom-up within the trophic levels of the food chain, as represented in the diagram below.
Bottom-Up and Top-Down Cascade Model for an Aquatic Ecosystem
Using evidence from the graphs and information provided, describe how the decision by humans to reintroduce the wolves into Yellowstone in 1995 had an impact on the environment of the area.
Base your answers to questions 14 through 18 on the information below and on your knowledge of biology.
Solving the Problem of Cat Allergies
Domestic cats are a very popular pet for humans. However, for 10–20% of people, cats can cause allergic reactions. Allergy symptoms are caused by exposure to an allergen, which can sometimes be a protein. Most cat allergy symptoms can be traced to the protein, Fel d 1. This protein is produced by the cells of various structures within the body of the cat, including salivary glands within the mouth and oil glands within the skin.
Information regarding the structure of Fel d 1 is included below.
Protein | Partial Amino Acid Sequence |
|---|---|
Fel d 1 (Allergen) | GLU TYR VAL GLU GLN VAL ALA GLN TYR LYS ALA LEU |
Base your answers to questions 19 through 22 on the information below and on your knowledge of biology.
A Hard Pest to Manage
The diamondback moth is an invasive species in many parts of New York State. The larval stage is an agricultural pest because it consumes vegetation and destroys crops such as cabbage, broccoli, and cauliflower. Chemical pesticides, such as butane-fipronil, can be used to control the effect of pests such as the diamondback moth. Some information about the diamondback moth is shown below.

The Effect of Butane-Fipronil on the Resistance Ratio of Diamondback Moths Over Time

Base your answers to questions 23 through 27 on the information below and on your knowledge of biology.
Diabetes
Diabetes is a chronic health condition that affects how the body regulates blood sugar (glucose). There are multiple forms of diabetes, including Type 1 and Type 2. Multiple symptoms can be used to diagnose diabetes. One of the symptoms associated with diabetes is muscle weakness and fatigue.
Base your answers to questions 28 through 32 on the information below and on your knowledge of biology.
Honeybees are social insects that live in large cooperative groups called hives. They are vital to the health of many ecosystems. As they eat pollen and nectar from flowers, they aid in the pollination of many flowering plants.
Honeybees exhibit group decision-making when they choose a new nesting site. Stressors such as a growing hive population, animal disruption, habitat fragmentation by humans, or a lack of available food will signal that the hive needs to find a new home. This causes the honeybees to engage in swarming behavior. Hives of up to 10,000 bees will send hundreds of scouts to find potential new sites, and they collectively select the best location.

Which solution could a community use to most effectively reduce the impact of human activity on the honeybee populations?
The Varroa mite is an invasive parasite of western honeybees. A mite infestation can cause a honeybee population to collapse. The mite feeds on both adult bees and larvae and can kill the larvae before they hatch. Adult bees try to protect their colony by cleaning out mites and removing infected bees.
Beekeepers treat heavily infested hives with oxalic acid, which kills Varroa mites. The data below show information about the number of mites in bee colonies before and after treatment with oxalic acid.
Oxalic Acid Varroa Treatment

Use the information provided to complete the diagram below by selecting the arrow in each box that describes the population trend that best supports the explanation that populations of bees are affected by mites and oxalic acid treatment.

Base your answers to questions 33 through 36 on the information below and on your knowledge of biology.
African baboons have been studied for their evolutionary relationships. Although all baboons are closely related and can reproduce, they are given separate species names due to the differences in their appearance, behaviors, and habitats. Since all baboon species can interbreed, genetic patterns have been closely examined in both the nuclear DNA (nDNA) and mitochondrial DNA (mtDNA). mtDNA is located in the mitochondria and is inherited only from the mother, who is more likely to remain in the geographic region of birth than males. The following diagrams show some information about African baboons and their biomes. Each letter indicates a different mtDNA group.
African Baboons

Map of Biomes in Africa

Describe the evidence from the graph that supports the claim that grooming increases an African baboon’s chance to survive and reproduce.
Base your answers to questions 37 through 40 on the information below and on your knowledge of biology.
Carnivorous plants are unique in the way they obtain and cycle compounds necessary for life functions. The purple pitcher plant (
The pitcher part of the plant is a modified hollow leaf that naturally fills with water. Insects are attracted to a sugary nectar secreted by the flared flap of the pitcher. The downward-pointing hairs lining the flap encourage insects to travel into the pitcher. The insects slide down into the fluid-filled main body of the pitcher and are digested by enzymes released by the plant.

Like most plants, the cells of pitcher plants are able to cycle carbon, as shown in the model below.
Model of the Processes of the Purple Pitcher Plant

Which statement describes how a process that occurs at X within the models results in the specialized cells seen in the fetus’s leg?
One of the 50-year-old twins, twin C, has been identified as being at risk for developing diabetes because a specific gene has been methylated. The same gene in twin D does not show signs of this change. How might genetic technology be used to prevent twin C from developing diabetes later in life?
Which question would help a student gather information to correctly show the role of DNA in the passing of traits from the original Hydrilla plant to the offspring that grow when broken off?
Which statement provides evidence from the mathematical representations provided and supports the claim that Hydrilla’s ability to efficiently capture resources will have negative effects on the biodiversity of the waterway in which they live?
Which solution would best control the Hydrilla population in bodies of water without harming native populations?
Which statement provides evidence that supports the claim that the complex interactions of bottom-up and top-down cascading play a role in maintaining relatively consistent numbers of organisms in a stable ecosystem?
Which statement best uses the process of carbon cycling in the biosphere to explain why phytoplankton function as a keystone species for this ecosystem?
Which statement would provide evidence that increased elk survival in Yellowstone is related to herd behavior?
Which statement supports the claim that the structure of DNA determines the structure of the Fel d 1 protein molecule produced by cat cells?
Based on the model above, describe how components of the immune system and respiratory system interact when a person with a cat allergy is exposed to Fel d 1.
A company recently came out with a new cat food that is treated with antibodies. They claim feeding this food to cats can reduce the amount of
Procedure Used to Reduce

Which statement explains the variation shown by week 10 in the amount of
Other researchers are using CRISPR-cas9 to solve the problem of allergy-causing cats. CRISPR-cas9 is a genetic technology that enables researchers to edit parts of an organism’s DNA by removing, adding, or altering sections of their DNA sequence. Researchers injected fertilized cat eggs with the extra DNA bases before any mitotic division. This prevented cells with the edited DNA from producing the functioning allergy-causing protein.
Which claim regarding the genetic makeup of the kittens produced by this technology is supported by the information provided?
Construct an explanation for how genes determine the structure of the Fel d 1 allergen by explaining why the use of CRISPR-cas9 to edit the cat’s DNA resulted in the inability of the salivary glands of cats to produce functional Fel d 1 protein.
Using evidence from the graph, which statement best explains the resistance ratio of diamondback moths?
Which question would a scientist ask to clarify the role of DNA in passing butane-fipronil resistance from one generation to the next?
Which statement best explains the variation in mortality observed in the two groups of diamondback moth larvae?
There are many mechanisms that cause pesticide resistance in insects. One mechanism changes the production of a protein that is needed for the transport of substances across the cell membrane. This occurs in both the diamondback moth and the European corn borer. The European corn borer can also develop resistance to
Adult European Corn Borer

Describe the scientific information that could be used to support the claim that the European corn borer and diamondback moth share a common ancestor.
Which model summarizes the process that could be disrupted by diabetes, resulting in muscle fatigue?
Using the model above, identify two body systems and describe how the systems interact to regulate glucose in an individual without diabetes.
Type 1 and Type 2 diabetes differ in their causes, onset, and management even though both result in issues with insulin. Type 1 diabetes is characterized by the reduced ability of the pancreas to produce insulin. Insulin is produced normally in Type 2 diabetes, but the cells of the body do not respond properly.
Glucose tolerance tests are used to diagnose issues with blood glucose regulation. Twenty study participants were asked to fast (not eat) before consuming 75 g of glucose. Their blood was drawn at intervals to assess the body’s response. The graphs below show some information from two participants in the study.
Use evidence to describe the role of insulin in a feedback mechanism that helps an individual maintain homeostasis in Participant 1 but not in Participant 2.
Proteins are made of one or more chains of amino acids. Bonds, such as disulfide bridges, can form between the amino acids of two separate chains to contribute to the structure of the protein. The model below shows some information about a segment of an insulin protein.
Insulin Protein Segment Model

Which evidence best supports the claim that a mutation would change the structure of insulin?
The management of diabetes has been improved through the use of technological advances. The following chart shows some information about possible management strategies for a person with diabetes.
Management Option | Description |
|---|---|
Personal injectables | Benefits
Risks
|
Insulin pump therapy | Benefits
Risks
|
Continuous glucose monitors (sensors) | Benefits
Risks
|
Pancreas transplant | Benefits
Risks
|
Analyze the given management strategies for a person with diabetes. State one criterion and one constraint that the scientists considered when developing these solutions to best meet the needs and wants of diabetics.
Based on the information above, how does the waggle dance of an individual bee have a beneficial effect for the hive?
Based on the data provided, which explanation best describes the effectiveness of using oxalic acid treatments every 18 days to reduce the number of invasive Varroa mites over time?
Using evidence and reasoning, evaluate the claim that the time of year for a Varroa mite treatment may determine whether a new ecosystem results in the following year.
State whether the claim is valid or not valid, and then explain your answer.
Which line of empirical evidence supports the claim that African baboons share a common ancestor?
Which evidence best explains how the evolution of African baboons is the result of an environmental factor?
Which statement provides evidence that explains how an environmental factor could cause a change in gene frequency for the likelihood of social behaviors in African baboons?
Using the model, which statement best identifies the energy conversion that occurs in the pitcher plant?
Which of these statements best predicts the movement of carbon between Earth’s spheres that produced the differences in carbon dioxide concentrations when the pitcher opens up?
How does this evidence support the scientists’ claim that increased nitrogen makes the pitcher plants more effective at cycling carbon?
Plants must obtain necessary compounds from their surrounding environment using specialized structures. Below is a representation of the process of nitrogen uptake occurring in non-carnivorous plants.
Plant Structures and Systems Involved in Nutrient Uptake

Using the model above, describe how components of the two systems interact in the non-carnivorous plant to obtain nitrogen from the surrounding environment that is necessary for its leaves to produce chlorophyll.
Base your answers to questions 41 through 45 on the information below and on your knowledge of biology.
A rare zebra coloration was discovered in Maasai Mara National Reserve in Kenya. The young plains zebra, named Tira, has black fur with white spots covering his body. His parents have the typical black and white stripe patterns usually seen in zebra species.

Which question would help scientists understand why the spotted zebra’s coat pattern is different from its striped parents?
Melanin is a pigment produced in the skin cells of mammals, including zebras. It gives mammal skin and fur its coloration. Melanocytes are specialized cells found in certain layers of the skin that produce this protein. The amount of pigment produced and the area where it deposits are some factors that determine the patterns of coloration in a zebra’s fur.
Melanin Production and Deposition

Describe evidence that would best support the explanation that spotted zebras and striped zebras have the same DNA sequence for their unique coloration patterns.
In the past, some scientists have suggested that stripes help zebras blend into their environment or that stripes are like a fingerprint that allow zebras to identify each other. Another hypothesis is that stripes prevent flies from landing on zebras, reducing the possibility of contracting a fatal disease carried by flies.
Researchers on a Kenyan savannah investigated whether flies prefer to land on striped or solid animal coats. The researchers measured how many flies landed on plains zebras, Grévy’s zebras, and solid-colored impalas.
The researchers claimed that an environmental factor may have affected the evolution of the zebra’s striped coat. Which row of the table uses evidence gathered by the researchers to justify why their claim is correct or incorrect?
Row | Claim | Reasoning |
|---|---|---|
1 | Correct | Flies are more attracted to the impala’s solid coat, resulting in more fl ies biting the zebras, and decreasing the zebras’ chance of surviving and reproducing. |
2 | Correct | Stripes reduce the number of biting fl ies landing on zebras, resulting in a decrease in the chance of disease from fl ies, and increasing the zebras’ chance of surviving and reproducing. |
3 | Incorrect | Stripes increase the number of biting fl ies landing on zebras, resulting in an increase in the chance of disease from fl ies, and decreasing the zebras’ chance of surviving and reproducing. |
4 | Incorrect | Flies are more attracted to the impala’s solid coat, resulting in fewer fl ies biting the zebras, and increasing the zebras’ chance of surviving and reproducing. |
The zebra’s natural habitat is in sub-Saharan Africa. Due to human activities such as agriculture and the building of roads and cities, zebra habitat is becoming increasingly fragmented or broken up. This isolates zebra populations from breeding with other populations.
In 2020, a group of scientists studied the genetic diversity of plains zebra populations in Africa.
Information about the frequency of the varied coat patterns in plains zebra populations is included in the table below.
Location | Plains Zebra Population Relative Size | Approximate Frequency of Varied Coat Patterns |
|---|---|---|
Etosha National Park, Namibia | Larger | 0.05% |
Lake Mburo National Park, Uganda | Smaller | 5% |
Which statement best uses the data gathered by the researchers to predict the variation and distribution of coat patterns in future generations of plains zebras?
The total population of the plains zebras has undergone an approximate 25% decline since 2002. The table below includes information on possible solutions to stabilizing and restoring zebra populations.
Solutions to Stabilize and Restore Zebra Populations
Solution | Summary |
|---|---|
Wildlife Corridors | Create protected pathways between habitats |
Sustainable Land Use Planning | Work with communities to limit development in key zebra habitats |
Protected Areas | Expand or establish conservation areas and reserves |
Identify a solution from the table and describe how it would reduce human impact on habitat fragmentation and benefit plains zebra populations.
Solution:
Description: