Enzymes are special types of proteins that help speed up chemical reactions. Remember: we said proteins help build things for our bodies. Some enzymes will break things into smaller pieces for our bodies to use, while others will put things together.
The enzyme we will be working with today is called catalase (found in the liver). Its function is to take hydrogen peroxide (a substrate) and break it down into oxygen gas and water (the products). If the enzyme catalase is working properly, you will see bubbles form as it breaks down the hydrogen peroxide.
What is the enzyme used in the experiment?
Label the chemical reaction that occurs with the hydrogen peroxide. Not all answers will be used.


When hydrogen peroxide is added to the Raw Liver sample, what do you think will happen? (Will there be a big reaction? Small reaction? No reaction at all?)
Answer in complete sentences or your score will be a 0 for this question.
When hydrogen peroxide is added to the Cooked Liver sample, what do you think will happen? (Will there be a big reaction? Small reaction? No reaction at all?)
Answer in complete sentences or your score will be a 0 for this question.

What was the function of the enzyme in the reaction?
What was breaking down the hydrogen peroxide and making the oxygen bubbles?
Which liver sample had enzymes that were denatured?
SELECT ALL THAT APPLY!!!
When a piece of liver is dropped into hydrogen peroxide, the peroxide bubbles vigorously.
What causes this reaction?
If the environment is too extreme, enzymes can be damaged under these conditions.
What condition affected the function of the enzyme in the lab?
Label the diagram below. Not all answers will be used.

The graphs show the reaction rate for an enzyme across a range of temperatures and pH.

Based on these data, this enzyme functions best at what temperature and pH?
What affect does changing temperature or pH have on an enzyme?
Beta-Galactosidase is an enzyme that breaks down galactose (a sugar). Whenever galactose is removed from Fluorescein, the molecule glows.

Normally, a certain species of jellyfish glows bright green. However, a small population was moved from the ocean into an aquarium, where the salinity (pH) is lower and can no longer glow.
The jellyfish no longer glow because the enzyme due to .
Hydrogen peroxide is a useful for disinfecting wounds. After scraping you knee hydrogen peroxide can be applied to
Normally, a certain species of jellyfish glows bright green. However, a small population was moved from the ocean into an aquarium, where the salinity (pH) is lower and can no longer glow.
The jellyfish no longer glow because the enzyme due to .
What do enzymes do?
What evidence would tell you whether the enzyme is working properly?
When hydrogen peroxide is added to the Pickled Liver sample, what do you think will happen? (Will there be a big reaction? Small reaction? No reaction at all?)
Answer in complete sentences or your score will be a 0 for this question.
When hydrogen peroxide is added to the Frozen Liver sample, what do you think will happen? (Will there be a big reaction? Small reaction? No reaction at all?)
Answer in complete sentences or your score will be a 0 for this question.
In test tube 1 with no liver, were produced because .
In test tube 2 with raw liver, were produced because .
In test tube 3 with cooked liver, were produced because .
In test tube 4 with pickled liver, were produced because .
In test tube 5 with frozen liver, were produced because .
The most bubbles were produced by the liver because this sample contained .