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Wednesday (02/26) - Post Lab and Denature

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Last updated about 3 hours ago
29 Nsɛmmisa
The Effect of Pectinase Lab
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Complete the table with your collected data. Complete the last page of your lab. When done turn in to my desk.

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Factors that Affect Enzymes
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Introduction:

Enzymes are proteins that act as catalysts in living cells. A catalyst speeds up a chemical reaction but is NOT used up or changed by the reaction. Enzymes catalyze almost all chemical reactions that occur in living organisms. Without the help of enzymes, chemical reactions in cells would take place too slowly. The molecule that the enzyme acts upon is called the substrate.

Pectin is a polysaccharide that functions as a stabilizing layer between adjoining cell walls of apples and other fruit. Pectinase is a naturally occurring enzyme that breaks down pectin the substrate. As an apple ripens, more pectinase is produced, which softens the fruit as the cell walls are weakened. Eventually, the cells break down and the juice is released.

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Asemmisa {{asɛmmisaAhyɛnsode}}
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Identify the enzyme used:

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Asemmisa {{asɛmmisaAhyɛnsode}}
7.

What color represents System A (no pectinase):

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Asemmisa {{asɛmmisaAhyɛnsode}}
9.

Label the following graph.

Mmuae Afoforo a Wobɛpaw:
Filtration System
Apple Juice Production (mL)
Time (min)
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10.

Label the following chemical reaction from your lab.

Mmuae Afoforo a Wobɛpaw:
Substrate
Enzyme
Product
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11.

Label the following chemical reaction from your lab.

Mmuae Afoforo a Wobɛpaw:
Pectin
Monosaccharide
Pectinase

Factors that affect enzymes

Things in the environment can change how well an enzyme will work!

What is pH?

Acids have a pH of 0-6

Neutral have a pH of 7

Bases have a pH of 8-15

Denaturing

Enzyme in the wrong temperature or pH lose their shape and DO NOT WORK ANYMORE.

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Asemmisa {{asɛmmisaAhyɛnsode}}
12.
  • pH 0-6

  • pH 7

  • pH 8-14

  • Battery Acid

  • Stomach

  • Bleach

  • Soap

  • Water

  • Brain

  • Acid

  • Neutraal

  • Bases

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13.

The figure A displays:

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14.

The figure B displays:

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15.

Which of the following can denature an enzyme? (SELECT ALL THAT APPLY!)

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16.

The optimal pH for enzymes in the stomach is . The enzymes in the human body function optimally at a temperature of

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19.

If enzyme A was placed into a pH of 8, which of the following would most likely occur?

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20.

Temperature & pH

What pH does enzyme B have the highest enzyme activity?

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21.

The optimal pH of this enzyme is .

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22.

The optimal pH of this enzyme is .

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23.

Identify the optimal temperature of the enzyme below.

Select your answer on the number line.

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24.

Enzyme A and Enzyme B would work equally well together at what pH?

Select your answer on the number line.

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25.

What happens to an enzyme's activity when you take it out of its optimal environment?

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26.

Enzymes are proteins that have a three-dimensional shape that is specific to a particular substrate. Environmental conditions can denature the shape of the enzyme.

If the shape of the enzyme changes, what will most likely happen?

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27.

Molecules that end with the suffix -ose are sugars. Molecules that end with the suffix –ase are enzymes.

Read the following names and decide if the molecule is an enzyme or is a sugar.

(Hint: There should be 4 molecules per category!)

  • Glucose

  • Polymerase

  • Lactose

  • Lactase

  • Enzymes (Select 2)

  • Sugars (Select 2)

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28.

Which biomolecule is lactose?

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29.

Which biomolecule is polymerase?

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2.

Identify the substrate used:

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3.

The carbohydrate used is .

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4.

An enzyme called amylase in the human mouth breaks down polysaccharides into monosaccharides. Monosaccharides are what the taste receptors on your tongue perceive as sweet.

Based on this information, how do you think breaking down the polysaccharide pectin (found in apples) will affect the taste of the apple juice?

Sentence stem:

The polysaccharide pectin will taste __________.

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5.

The x-axis description and units

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6.

The y-axis description and units

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8.

What color represents System B (with pectinase):