Spring til hovedindhold
Log ind
Sign up for FREE
arrow_back
Bibliotek

Wednesday (02/26) - Post Lab and Denature

star
star
star
star
star
Sidst opdateret 20 days ago
29 Spørgsmål
The Effect of Pectinase Lab
Nødvendig
1

Complete the table with your collected data. Complete the last page of your lab. When done turn in to my desk.

Nødvendig
1
Nødvendig
1
Factors that Affect Enzymes
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1

Introduction:

Enzymes are proteins that act as catalysts in living cells. A catalyst speeds up a chemical reaction but is NOT used up or changed by the reaction. Enzymes catalyze almost all chemical reactions that occur in living organisms. Without the help of enzymes, chemical reactions in cells would take place too slowly. The molecule that the enzyme acts upon is called the substrate.

Pectin is a polysaccharide that functions as a stabilizing layer between adjoining cell walls of apples and other fruit. Pectinase is a naturally occurring enzyme that breaks down pectin the substrate. As an apple ripens, more pectinase is produced, which softens the fruit as the cell walls are weakened. Eventually, the cells break down and the juice is released.

Nødvendig
1
Spørgsmål 1
1.

Identify the enzyme used:

Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Nødvendig
1
Spørgsmål 7
7.

What color represents System A (no pectinase):

Nødvendig
1
Spørgsmål 9
9.

Label the following graph.

Andre svarmuligheder:
Filtration System
Apple Juice Production (mL)
Time (min)
Spørgsmål 10
10.

Label the following chemical reaction from your lab.

Andre svarmuligheder:
Substrate
Enzyme
Product
Spørgsmål 11
11.

Label the following chemical reaction from your lab.

Andre svarmuligheder:
Pectin
Monosaccharide
Pectinase

Factors that affect enzymes

Things in the environment can change how well an enzyme will work!

What is pH?

Acids have a pH of 0-6

Neutral have a pH of 7

Bases have a pH of 8-15

Denaturing

Enzyme in the wrong temperature or pH lose their shape and DO NOT WORK ANYMORE.

Nødvendig
1
Spørgsmål 12
12.
  • pH 0-6

  • pH 7

  • pH 8-14

  • Battery Acid

  • Stomach

  • Bleach

  • Soap

  • Water

  • Brain

  • Acid

  • Neutraal

  • Bases

Spørgsmål 13
13.

The figure A displays:

Spørgsmål 14
14.

The figure B displays:

Spørgsmål 15
15.

Which of the following can denature an enzyme? (SELECT ALL THAT APPLY!)

Spørgsmål 16
16.

The optimal pH for enzymes in the stomach is . The enzymes in the human body function optimally at a temperature of

Spørgsmål 19
19.

If enzyme A was placed into a pH of 8, which of the following would most likely occur?

Spørgsmål 20
20.

Temperature & pH

What pH does enzyme B have the highest enzyme activity?

Spørgsmål 21
21.

The optimal pH of this enzyme is .

Spørgsmål 22
22.

The optimal pH of this enzyme is .

Spørgsmål 23
23.

Identify the optimal temperature of the enzyme below.

Select your answer on the number line.

Spørgsmål 24
24.

Enzyme A and Enzyme B would work equally well together at what pH?

Select your answer on the number line.

Spørgsmål 25
25.

What happens to an enzyme's activity when you take it out of its optimal environment?

Spørgsmål 26
26.

Enzymes are proteins that have a three-dimensional shape that is specific to a particular substrate. Environmental conditions can denature the shape of the enzyme.

If the shape of the enzyme changes, what will most likely happen?

Spørgsmål 27
27.

Molecules that end with the suffix -ose are sugars. Molecules that end with the suffix –ase are enzymes.

Read the following names and decide if the molecule is an enzyme or is a sugar.

(Hint: There should be 4 molecules per category!)

  • Polymerase

  • Lactase

  • Lactose

  • Glucose

  • Enzymes (Select 2)

  • Sugars (Select 2)

Spørgsmål 28
28.

Which biomolecule is lactose?

Spørgsmål 29
29.

Which biomolecule is polymerase?

Spørgsmål 2
2.

Identify the substrate used:

Spørgsmål 3
3.

The carbohydrate used is .

Spørgsmål 4
4.

An enzyme called amylase in the human mouth breaks down polysaccharides into monosaccharides. Monosaccharides are what the taste receptors on your tongue perceive as sweet.

Based on this information, how do you think breaking down the polysaccharide pectin (found in apples) will affect the taste of the apple juice?

Sentence stem:

The polysaccharide pectin will taste __________.

Spørgsmål 5
5.

The x-axis description and units

Spørgsmål 6
6.

The y-axis description and units

Spørgsmål 8
8.

What color represents System B (with pectinase):