Copy of Friday 2/6 Electrophoresis practice (5/2/2026) - Nancy Morfin | Bibliotek

Nødvendig

1

Nødvendig

1

Nødvendig

1

Nødvendig

1

Nødvendig

1

Nødvendig

1

Nødvendig

1

null

Nødvendig

3

Study the illustration of children and parent DNA bands and determine the nature of the relationship between each child and the parents.

Draggable emne		Korresponderende emne
child 2		child with two natural parents
child 3		child with adoptive parents
child 1		child with natural mother and adoptive father

Nødvendig

1

Compare the DNA from 4 suspects with the DNA obtained from saliva found on a cigarette butt left at a crime scene. Select the match.

Scenario

A high school was broken into, and several computers were stolen. Police were called when a motion alarm went off. They found a door that looked locked but had been propped open with paper. Near the door, they found a cold soda can and believed it was left by one of the suspects.

Police collected DNA from the soda can. A nearby store clerk remembered selling soda to three young men before the break-in. The men were identified, fingerprinted, and gave DNA samples to help police find out who was involved.

Nødvendig

1

What is the purpose of the DNA ladder?

Nødvendig

1

Did any suspect share all four bands of DNA with the crime-scene DNA? If so, which suspect or suspects?

Nødvendig

1

Note that one band of the evidence DNA was found in all four suspects. Explain how this is possible.

Nødvendig

1

What does electrophoresis primarily separate in a mixture?

Atoms by their atomic mass

Molecules based on size and charge

Liquid from solid residue

Molecules by their boiling point

Nødvendig

1

What type of samples are being analyzed using electrophoresis?

lipids

nucleic acids

carbohydrates

waxes

Nødvendig

1

Which direction do negatively charged molecules move in electrophoresis?

In random directions

Towards the negative electrode

They do not move at all

Towards the positive electrode

Nødvendig

1

In agarose gel electrophoresis, what direction do DNA fragments move?

Only larger fragments move.

No net movement occurs.

Towards the positive electrode.

Towards the negative electrode.

Nødvendig

1

What is the primary function of restriction enzymes?

Transcribe RNA from DNA

Cut DNA at specific sequences

Join DNA fragments together

Synthesize DNA strands

Nødvendig

1

How do restriction enzymes aid gel electrophoresis?

They improve DNA replication speed

They produce DNA fragments of varying sizes

They stain the gel for visualization

They power the electrophoresis process

Nødvendig

1

What happens to DNA after restriction enzyme digestion?

It is separated by size in gel electrophoresis

It evaporates into the air

It is converted into RNA

It becomes larger and heavier

Nødvendig

1

Why is DNA amplification necessary before electrophoresis?

To purify the DNA from contaminants

To obtain enough DNA for analysis

To shorten the DNA length

To change the DNA sequence

Nødvendig

1

Which process is being utilized during PCR

Transcription

DNA Replication

Translation

Mutation