NOTICE … A four-function or scientific calculator must be available for you to use while taking this examination. Note that diagrams are not drawn to scale unless otherwise noted.
NOTICE … A four-function or scientific calculator must be available for you to use while taking this examination. Note that diagrams are not drawn to scale unless otherwise noted.
Base your answers to questions 1 through 5 on the information below and on your knowledge of Life Science: Biology. Be sure to record your answers for multiple-choice questions on the separate answer sheet provided.
A Seasonal Strategy for Survival
Certain species of organisms living in colder climates have evolved unique adaptations to conserve energy during winter months. These organisms enter a state of hibernation, a period of inactivity that causes life processes to slow down. It has been estimated that this period of inactivity allows organisms to use nearly 70 to 100 times less energy when resources are limited. Some species of ground squirrels hibernate.
The model below includes some components of squirrel body systems.
Anatomy of a Ground Squirrel

Researchers studying animals that hibernate discovered that some of the physiological changes associated with hibernation could also lead to the development of blood clots. These animals had a significant difference in their platelet count when hibernating, compared to when they were not hibernating.
Platelets are a component of blood that are formed within the marrow of bones, such as those in the legs. They help with the clotting of blood.
The information below summarizes some of the changes that occur in hibernating animals.
Sample Physiological Changes in Hibernating Animals

Base your answers to questions 6 through 9 on the information below and on your knowledge of Life Science: Biology.
Nitrogen: Friend or Foe?
Algae are simple, plant-like organisms that play an essential role in many aquatic ecosystems. However, not all algae growth is beneficial to the ecosystem.
Researchers tested the effect of nitrogen (N) on algae growth by adding varying amounts of nitrogen to water samples containing algae. The data they collected are shown in the graphs that follow.

Scientists claim that 144 mg/L of nitrogen can negatively impact the complex interactions between organisms in freshwater ecosystems. Evaluate whether or not this claim is valid by writing Valid or Not valid and provide data to support your evaluation.
Valid or Not valid:
Explanation:
Base your answers to questions 15 through 18 on the information below and on your knowledge of Life Science: Biology.
Polycystic Ovary Syndrome (PCOS)
Human egg cells are highly specialized for the function of sexual reproduction. Egg cells experience a sequence of changes coordinated by the interactions between organs, glands, and hormones.
Polycystic Ovary Syndrome (PCOS) is a disorder that affects 8–13% of women of reproductive age. Many women with PCOS find it more challenging to get pregnant than women without PCOS.
The model below represents the female reproductive cycle over 28 days.

Use information from the model to describe how a specific hormone could affect a structure of the female reproductive system.
Base your answers to questions 19 through 22 on the information below and on your knowledge of Life Science: Biology.
Tailless Humans
Humans have a large percentage of DNA in common with other primates. One group, referred to as hominoids, diverged from monkey-like primates 15–20 million years ago (Mya) and evolved without tails, as shown in the model below.
Primate Evolutionary Tree
Scientists have identified a gene,
Base your answers to questions 23 through 27 on the information below and on your knowledge of Life Science: Biology.
Fossil Fuels: Out of Sight, but Not Out of Mind
The Carboniferous Period, 359 to 299 million years ago, was characterized by a rapid increase in plant biomass. Incredibly dense forests covered large areas of Earth’s tropical regions. The matter stored in the biomass of these forests underwent various processes. The chemical processes at work today are the same that were present on ancient Earth.
The diagram below represents some interactions between components of the Earth’s spheres.
Formation of Fossil Fuels

Construct an explanation, based on evidence, for how reproduction contributes to the process of evolution in honeybees.
Construct an explanation based on evidence for why the cycling of carbon in the forest may change, resulting in an increase in carbon dioxide levels as the tapir population decreases.
Base your answers to questions 38 through 41 on the information below and on your knowledge of Life Science: Biology.
Caffeine is a natural compound found in over 60 plants. Many of these plants are used in food products. However, labels do not always list the amount of caffeine in a food product, making it difficult to know exactly how much caffeine is being consumed per day.
The side effects of caffeine consumption can include increased anxiety, inability to sleep, heart palpitations, irritability, dehydration, and digestive issues like nausea.
One organization of pediatric doctors suggests that teenagers between the ages of 12–18 should not consume more than 100 mg of caffeine per day. Unfortunately, many teenagers and adults far exceed this limit due to the increased popularity of energy drinks that can contain up to 300 mg of caffeine.
The diagram below shows some of the body systems that are affected by caffeine.

Construct an explanation based on the evidence provided for how the structure of the
Base your answers to questions 42 through 45 on the information below and on your knowledge of Life Science: Biology.
Australia is a uniquely biodiverse continent that has many marsupials, a type of mammal with a pouch to carry their young. Australia was once part of a supercontinent that was home to the earliest marsupials between 178 and 168 million years ago. Australia began to separate from the larger land mass about 130 million years ago, taking its marsupials along with it. When a mass extinction occurred about 65 million years ago, conditions on Earth changed, allowing placental mammals to take over most ecosystems except in Australia, where marsupials continue to dominate to this day.
The genome of the Tasmanian wolf, an extinct marsupial, is being analyzed to determine how it is related to modern-day marsupials. Specific sections of the genome that contained repeated insertions were analyzed. These segments included the
Some information about marsupials is provided below.

Using the evidence provided, construct an explanation about why the modern-day koala was able to survive while the giant koala went extinct.
Base your answers to questions 46 through 49 on the information below and on your knowledge of Life Science: Biology.
Onondaga Lake is located in the city of Syracuse, New York. Beginning in the 1880s, an industrial company near the lake started dumping mercury-contaminated waste products directly into Onondaga Lake. This dumping continued until 1970. During this time period, fishing and swimming were banned due to safety concerns regarding the effect of mercury on the health of organisms. Remediation and cleanup of the lake is part of an ongoing land restoration effort that includes the implementation of long-term maintenance and monitoring programs to ensure the remedy’s effectiveness. The diagram below shows different ways that pollution can enter a lake.

Which statement best describes the interactions between body systems found in the Anatomy of a Ground Squirrel model that are used to protect the squirrel from blood clot formation during hibernation?
Using the data compiled by the researchers, provide evidence that supports the claim that as environmental temperature drops, feedback mechanisms maintain homeostasis in hibernating animals.
Which model below best represents the inputs and outputs of the chemical process by which matter and energy are converted into forms that are usable for the ground squirrel cells?
During hibernation studies, researchers have also found that the function of the immune system may be affected in mammals.
The chart below shows the data collected from the investigations.
Approximate White Blood Cell Reduction During Hibernation
Animal | Percent Reduction |
|---|---|
Scandinavian Brown Bear | 40% |
13-Lined Ground Squirrel | 90% |
Northern Long-Eared Bat | 45% |
Given the data provided, which explanation best identifies how changes in the immune system allow animals, such as squirrels and bears, to maintain homeostasis during hibernation?
Construct an explanation using evidence that bats that awaken periodically will increase in the population as a result of the process of evolution.
Using data from the graphs, which factor has the greatest effect on the size of the algae population?
Scientists claim that the addition of low amounts of nitrogen will affect the carrying capacity of all of the species in the entire food web. Using the given information, which explanation best supports the scientists’ claim?
Which solution would most effectively and safely reduce the chances of harmful algae blooms caused by human activity?
Base your answers to questions 10 through 14 on the information below and on your knowledge of Life Science: Biology.
In 1986, a nuclear reactor in Chernobyl, located in Eastern Europe, released a massive amount of radioactive material into the atmosphere. The surrounding area remains largely uninhabitable by humans to this day. The released radiation will take many years to naturally return to safe levels.
The Eastern tree frog (
Color Variation in H. orientalis

Which question best clarifies the role of DNA in causing a change in the inheritance of skin color in frogs?
Which evidence would best support the claim that color distribution and frequency in the frog population before the Chernobyl disaster was the result of natural selection?
Which evidence from the information provided shows that the radiation released into the environment around Chernobyl caused a change in pigmentation in the Eastern tree frog population?
After the release of radiation, the populations of animals per square kilometer (No./km²) near the Chernobyl site were monitored for several years. The populations are now similar in size to protected nature reserve populations not affected by the Chernobyl radiation, with some populations being larger than in uncontaminated areas. The studies showed no correlation between radiation levels and the relative abundance of the animals studied in an area. The graph below shows the data for three animal species in the first few years after the disaster.
Which claim about the complex interactions of the animal populations in Chernobyl is best supported by the data after the release of the radiation?
Immediately after the Chernobyl disaster, the roe deer population was reduced to almost zero. Construct an explanation as to how natural selection led to the observed increase in the roe deer population after the Chernobyl disaster.
Which statement identifies evidence that supports the claim that PCOS is the result of a failure of a feedback mechanism associated with the egg cell?
Once an egg is released, several mechanisms within the egg ensure that only one sperm contributes genetic information to the zygote. The binding of the sperm with the egg causes the endoplasmic reticulum (ER), an organelle within the egg, to release signals causing changes within the egg. This prevents additional sperm from binding to the egg. The model below represents one proposed mechanism.
Which statement correctly pairs a structure in the model with a function that leads to the formation of the impenetrable membrane?
In addition to increased difficulty in becoming pregnant, PCOS can lead to non-reproductive symptoms. Researchers are investigating strategies that can be used to treat the symptoms of PCOS. Two of these treatment plans are described below.
Plan 1: Medication to manage specific symptoms.
Plan 2: Exercise and improved diet to reduce severity of symptoms.
Identify which of the given treatment plans would most effectively reduce the symptoms of PCOS. You may consider two of the following constraints: cost, safety, or reliability. Complete the table to record your response.
Plan Number:
Constraint | Trade-off | Reason Why |
|---|---|---|
Pro | ||
Con |
Which statement provides empirical evidence that could be used to determine the placement of chimps and humans in the evolutionary tree diagram?
Which statement contains evidence that best supports the explanation that the structure of the
Which question about the
A claim was made that variations produced by genetic engineering are inherited by mouse offspring. Provide evidence from the data to support this claim.
Based on the model, which explanation best describes the role of one process involved in the cycling of carbon that led to the formation of fossil fuels?
Use the evidence provided to support an explanation for why the carrying capacity for plants was much different during the Carboniferous Period than today.
Which statement supports the argument that the coevolution of feedbacks between living things and Earth’s systems occurred during the Carboniferous Period?
Use quantitative data from the model to describe how the cycling of carbon between the atmosphere and hydrosphere has been changed by the actions of humans.
Which statement best describes a criterion or constraint accounting for societal needs that must be considered when designing a solution to the overuse of fossil fuels?
Base your answers to questions 28 through 32 on the information below and on your knowledge of Life Science: Biology.
Buzzy Bees
Honeybees play an essential role in providing ecosystem services for humans. Honeybees alone pollinate 80% of all flowering plants, including more than 130 types of fruits and vegetables. The group behaviors that honeybees employ make them effective at pollination. These bees are especially good at defending their hive and making sure they can provide honey for all the hive members.
Honeybees are social insects that live in colonies, including a queen, thousands of worker bees, and male drones. In spring and summer, young worker bees gather pollen and nectar and defend the colony. Honeybees survive the winters by using nectar and pollen to produce honey.
Two processes shown below are ongoing in the environments in which hives are present.

Which explanation best supports the claim that honeybees play a role in the flow of energy in an ecosystem?
Colonies of honeybees consist of members that have different roles within the hive, as indicated in the table below. The diagram at the bottom shows the possible outcomes of mating within a hive.
Role of Types of Honeybees
Type | Roles Within the Hive |
|---|---|
Queen
|
|
Drones
|
|
Workers
|
|
Results of Queen Mating

Which statement identifies evidence that best supports the role of bees using group behavior to increase the survival of the honeybee hive?
Which claim is supported by the evidence that a change in the honeybees’ environment may lead to its extinction?
Bees may not be present in the areas where they are needed for pollination. Without bees, the food supplies available to humans would be greatly limited. Beekeepers securely transport their hives great distances in trucks to provide pollination services.
The map and table provide some information about the transportation of honeybee colonies.
Honeybee Colony Movements into California, July 1, 2017–Jan. 1, 2018

Advantages and Disadvantages to Transporting Honeybee Colonies
Advantages | Disadvantages |
|---|---|
|
|
Evaluate whether transporting hives is an effective solution to pollinate crops for human consumption by indicating Effective or Not effective.
Support your evaluation using the specific criteria and trade-offs of cost, safety, and environmental impact. Indicate your choice and justification in your response.
Start your answer with "Effective" or "Not effective" before going into your explanation.
Base your answers to questions 33 through 37 on the information below and on your knowledge of Life Science: Biology.
Tapirs are a type of mammal that is native to tropical forests. One species, the Baird’s tapir, lives in the jungles of Costa Rica in Central America. They have existed for at least 50 million years and are most closely related to the horse and rhinoceros.
Tapirs consume a wide variety of plants and fruit. They play a vital role in their ecosystem as “gardeners of the forest,” contributing to forest regeneration and nutrient cycling. Tapirs eat the fruit of trees, releasing the seeds from the fruit in their feces. This disperses the seeds throughout the forest, as shown in the diagram below.
One study found that the tapir’s role in forest regeneration is even more vital when a forest has been degraded (negatively impacted).
Tapir populations have been declining in Central America due to their low reproductive rate, habitat destruction, and poaching (illegal hunting). They are now listed as an endangered species.

Scientists claim that maintaining the Baird’s tapir is important because they contribute to the biodiversity of the rainforest ecosystem. In 1929, several tapirs were reintroduced to an island in Central America in an effort to reestablish a population there. Data were collected over the next 60 years to monitor the trends in the Baird’s tapir population on the island.
The graph below shows some information about the Baird’s tapir population and related human activities.
Baird’s Tapir Population Trends in Central America

Which statement could be used to evaluate the solutions used by the scientists to reduce the impact of humans on this population of Baird’s tapir?
Which statement identifies a claim that explains why there would be more Baird’s tapirs than jaguars in this rainforest ecosystem?
The claim has been made that the complex interactions required to maintain ecosystem stability would have to be changed if the overall trend observed between 1930–1990 in the tapir population continued. State your evaluation of the claim and use evidence from the Energy Transfer in a Rainforest Ecosystem model and the graph to support your evaluation.
Evaluation:
Support with Evidence:
Which statement correctly identifies an explanation for the flow of energy in this forest ecosystem?
Which statement best describes interactions between two body systems represented in the model that allow caffeine to be utilized in the body?
The ancestral form of the
Which claim best explains a cause of the recent inheritable variation in the
When caffeine binds to the adenosine receptor, it is primarily digested by an enzyme, CYP1A2. Which question could be asked to best clarify how DNA codes for the formation of the CYP1A2 enzyme and is passed from parents to offspring?
Which statement provides evidence about common ancestry in marsupials?
Which claim about the effect of changing environmental conditions is supported by the evidence provided?
Koalas are another marsupial native to Australia with a unique diet of mostly eucalyptus leaves. Eucalyptus contains chemicals that are toxic to most animals. Koalas have a specialized digestive system and a slow metabolism that allows them to consume eucalyptus. The giant koala was a close relative of the modern-day koala, but it did not survive to present day. The table below shows some information about koalas.
History of Koala Species
Species | Approximate Temporal Range | Environment | Diet | Average Weight (kg) |
|---|---|---|---|---|
Giant koala | 15 million–12,000 years ago |
| Mainly eucalyptus leaves | 15–20 |
Modern-day koala | 25 million years ago–Present day |
| Only eucalyptus leaves | 4–15 |
Since the late 1800s, scientists have documented an increase in the size of some structures that function to regulate temperature in several Australian animals. For example, appendages such as the beaks of parrots, tails of shrews, and wings of bats are longer in the present day than in the past.
The graph below shows an environmental change that occurred during the same time frame.
Which explanation about the change in appendages of several Australian animals is supported by evidence?
Which solution would further reduce the impact of human activities on the Onondaga Lake environment and its biodiversity?
Description | Mercury Concentration ( |
|---|---|
Predators (walleye, smallmouth) | 10,000,000 |
Medium-sized fish (pumpkinseed) | 1,000,000 |
Minnows (banded killifish) | 100,000 |
Fish frye (young) | 10,000 |
Insect larvae | 1000 |
Protozoa & zooplankton | 100 |
Bacteria & phytoplankton | 10 |
Water | 1 |
Based on the mathematical information in the diagram and the table, which statement explains why top predators are particularly vulnerable to mercury contamination in Onondaga Lake?
Use the data provided to evaluate the claim that the remediation program has affected the complex interactions in the Onondaga Lake ecosystem and could result in a change in the predator species population size.
Fishermen have been warned not to eat some of the fish they catch in Onondaga Lake. Health officials are trying to come up with an effective plan to supply the information to the public in order to prevent these harmful fish from being consumed.
Criteria:
Must reach as many people that are fishing as possible.
Must be reliable—inform people so they make the choice not to eat the fish.
Constraints:
Must cost as little as possible
Must not require staff to be available at all times
Below is a table of possible solutions.
Solutions | Considerations |
|---|---|
1 - Flyers | – Would provide valuable information to fishermen – Staff would need to pass out flyers – Easily discarded/ignored |
2 – Signs at boat launches/piers | – Would provide valuable information to fishermen – No staff needed after signs are posted – Only some fishermen will take the time to read the information |
3 – Law enforcement boats | – Staff would provide reliable information to each individual that they speak to – Would be able to identify fishermen who are at risk – Staff would be needed to patrol on a consistent basis |
Identify one solution from the table to effectively educate fishermen on the health concerns of eating fish that contain dangerous levels of mercury and explain how it meets the given criteria and constraints.
Solution:
Explanation: