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Cytochrome C Comparison Lab

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Last updated over 6 years ago
29 questions
Note from the author:
Ottawa High School Curriculum 2019 Biology textbook McDougal Littell ed 2008
Instructions
Student Worksheet - pg 1 of 2 (open Box #2 to enter numbers into Table 1) We will share data collection. Each student will be given one of the pairs of organisms to count. Data will then be shared.
Student Worksheet - pg 2 of 2
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Question 1
1.

Last Name First Name

enter your name
Question 2
2.

Count the number of differences between each of the organisms cytochrome C amino acid sequence and place the number in the appropriate box in the table. See Instructions--> Method--> A--> numbers 1-4 for an example. We will count these as a class and put them on a shared google document. It will be easier for you to enter the data at one time after you have it on the class shared document.

Question 3
3.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

Question 4
4.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

Question 5
5.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

Question 6
6.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

Question 7
7.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

Question 8
8.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

Question 9
9.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

Question 10
10.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

Question 11
11.

type the organism's name in lower case letters, use the organism name that is listed in Table 1

Question 12
12.

this is a node, enter the number of differences from your Table 1 between these organisms (#10 and #11)

Question 13
13.

this is a node, enter the number of differences from your Table 1 between these organisms

Question 14
14.

this is a node, enter the number of differences from your Table 1 between these organisms (#7 &#8)

Question 15
15.

this is a node, enter the number of differences from your Table 1 between these organisms (#8 & #9)

Question 16
16.

this is a node, enter the number of differences from your Table 1 between these organisms (#6 & #7)

Question 17
17.

this is a node, enter the number of differences from your Table 1 between these organisms (#5 & #6)

Question 18
18.

this is a node, enter the number of differences from your Table 1 between these organisms (#3 & #5 or #4 & #5); there is more than one correct answer, however, enter only one of the numbers

Question 19
19.
Look at the set of amino acid sequences you used to collect data
Question 20
20.

enter the animal's name that goes on this branch; use lower case letters

Question 21
21.

enter the name of the animal that goes on this branch; use only lower case letters

Question 22
22.

enter the name of the organism that belongs on this branch; use only lower case letters

Question 23
23.

enter a number

Question 24
24.

enter a number

Question 25
25.

I used personal knowledge. I have seen a picture of a live horse, donkey and zebra. All of them have similar body structures and look and act "horsey."

Question 26
26.

The branch should be added

The fewer the differences in cytochrome C amino acids indicates the organisms are more closely related.
Question 27
27.

List 3 things you used to determine how organisms are related to each other.

This lab is an indirect DNA analysis.
Question 28
28.

Select whether this a true or false statement to answer Question #6:
More closely related species have more recent common ancestors, therefore there has been less time for DNA mutations to occur and so their DNA is very similar and has less differences.

Question 29
29.

Select whether this is a true or false answer to Question #7:

This cytochrome C data indicates that plants and fungi originated off of the same node because they both have similar differences in sequences compared to all of the other animals. Animal life begins off of the next node (more recent) on the cladogram, so both plants and fungi are equal distance from the first node/branch of animals.

Wheat is the plant and yeast is the fungi in this study. All of the other organisms in this study are animals (including the moth). The differences of sequences is in the 40's for both yeast and wheat compared to all of the other organisms.